1921
Volume 90, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

, the causative agent of Chagas' disease, preferentially infects cardiac and digestive tissues. Baboons living in Texas () and cynomolgus monkeys (s) have been reported to be infected naturally with . In this study, we retrospectively reviewed cases of animals that were diagnosed with lymphocytic myocarditis and used a polymerase chain reaction (PCR)-based method (S36/S35 primer set) to amplify DNA from archived frozen and formalin-fixed paraffin-embedded (FFPE) cardiac tissues. We show that the PCR method is applicable in archived frozen and FFPE tissues and the sensitivity is in the femtogram range. A positive correlation between PCR positivity and lymphocytic myocarditis in both baboons and cynomolgus monkeys is shown. We also show epicarditis as a common finding in animals infected with .

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2014-04-02
2017-09-24
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  • Received : 31 Jul 2013
  • Accepted : 18 Jan 2014

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