Volume 90, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



causes melioidosis, the third most common cause of death from infectious diseases in northeast Thailand. Four physicochemical factors were set so that their values covered the range of the northeast, which is an endemic area. The soil pH was set at pH 4–10, soil salinity was 0.0–5.0% NaCl, total iron was 50–150 mg/kg soil, and carbon to nitrogen ratio (C/N) was 10:1 to 40:1. The experiments were carried out at 37°C, and soil moisture was maintained for 7 days. The number of viable bacterial cells was counted daily. Soil pH, salinity, Fe, and C/N ratio affected the bacterial growth. The bacterial colony was significantly ( < 0.05) reduced at soil pH > 8, soil salinity > 1% NaCl, and C/N ratio > 40:1. However, the growth of was enhanced by increasing the concentrations of iron significantly ( < 0.05). We propose using these findings to control .


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  1. Leelarasamee A, , 2000. Melioidosis in Southeast Asia. Acta Trop 74: 129132.[Crossref]
  2. White NJ, , 2003. Melioidosis. Lancet 361: 17151722.[Crossref]
  3. Centers for Disease Control and Prevention, 2013. Bioterrorism Agents/Diseases. Available at: http://www.bt.cdc.gov/agent/agentlist-category.asp. Accessed November 15, 2013.
  4. Currie BJ, Mayo M, Anstey NM, Donohoe P, Haase A, Kemp DJ, , 2001. A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates. Am J Trop Med Hyg 65: 177179.
  5. Currie BJ, Ward L, Cheng AC, , 2010. The epidemiology and clinical spectrum of melioidosis: 540 cases from the 20 year Darwin prospective study. PLoS Negl Trop Dis 4: e900.[Crossref]
  6. Inglis TJ, Garrow SC, Adams C, Henderson M, Mayo M, Currie BJ, , 1999. Acute melioidosis outbreak in Western Australia. Epidemiol Infect 123: 437443.[Crossref]
  7. Yeager J, Facemire P, Dabisch P, Robinson C, Nyakiti D, Beck K, Baker R, Pitt M, , 2012. Natural history of inhalation melioidosis in rhesus macaques (Macaca mulatta) and African green monkeys (Chlorocebus aethiops). Infect Immun 80: 33323340.[Crossref]
  8. Allworth AM, , 2005. Tsunami lung: a necrotising pneumonia in survivors of the Asian tsunami. Med J Aust 182: 364.
  9. Chierakul W, Winothai W, Wattanawaitunechai C, Wuthiekanun V, Rugtaengan T, Rattanalertnavee J, Jitpratoom P, Chaowagul W, Singhasivanon P, White NJ, Day NP, Peacock SJ, , 2005. Melioidosis in 6 tsunami survivors in southern Thailand. Clin Infect Dis 41: 982990.[Crossref]
  10. Ender P, Dolan M, , 1997. Pneumonia associated with near-drowning. Clin Infect Dis 25: 896907.[Crossref]
  11. Cheng AC, Currie BJ, , 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18: 383416.[Crossref]
  12. Dance DA, , 1991. Melioidosis: the tip of the iceberg? Clin Microbiol Rev 4: 5260.[Crossref]
  13. Dharakul T, Songsivilai S, , 1999. The many facets of melioidosis. Trends Microbiol 7: 138140.[Crossref]
  14. Smith MD, Wuthiekanun V, Walsh AL, White NJ, , 1995. Quantitative recovery of Burkholderia pseudomallei from soil in Thailand. Trans R Soc Trop Med Hyg 89: 488490.[Crossref]
  15. Boonsawat W, Boonma P, Tangdajahiran T, Paupermpoonsiri S, Wongpratoom W, Romphryk A, , 1990. Community-acquired pneumonia in adults at Srinagarind Hospital. J Med Assoc Thai 73: 345352.
  16. Chaowagul W, White NJ, Dance DA, Wattanagoon Y, Naigowit P, Davis TM, Looareesuwan S, Pitakwatchara N, , 1989. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis 159: 890899.[Crossref]
  17. Vuddhakul V, Tharavichitkul P, Na-Ngam N, Jitsurong S, Kunthawa B, Noimay P, Binla A, Thamlikitkul V, , 1999. Epidemiology of Burkholderia pseudomallei in Thailand. Am J Trop Med Hyg 60: 458461.
  18. Leelarasamee A, Trakulsomboon S, Kusum M, Dejsirilert S, , 1997. Isolation rates of Burkholderia pseudomallei among the four regions in Thailand. Southeast Asian J Trop Med Public Health 28: 107113.
  19. Ashdown LR, Duffy VA, Douglas RA, , 1980. Meliodosis. Med J Aust 1: 314316.
  20. Currie BJ, Fisher DA, Howard DM, Burrow JN, Selvanayagam S, Snelling PL, Anstey NM, Mayo MJ, , 2000. The epidemiology of melioidosis in Australia and Papua New Guinea. Acta Trop 74: 121127.[Crossref]
  21. Hanna JN, Humphreys JL, Brookes DL, Messina T, Raulli A, , 2010. Melioidosis in north Queensland, 2000–2009. Commun Dis Intell 34: 444447.
  22. Malczewski AB, Oman KM, Norton RE, Ketheesan N, , 2005. Clinical presentation of melioidosis in Queensland, Australia. Trans R Soc Trop Med Hyg 99: 856860.[Crossref]
  23. Limmathurotsakul D, Wongratanacheewin S, Teerawattanasook N, Wongsuvan G, Chaisuksant S, Chetchotisakd P, Chaowagul W, Day NP, Peacock SJ, , 2010. Increasing incidence of human melioidosis in Northeast Thailand. Am J Trop Med Hyg 82: 11131117.[Crossref]
  24. Limmathurotsakul D, Jamsen K, Arayawichanont A, Simpson JA, White LJ, Lee SJ, Wuthiekanun V, Chantratita N, Cheng A, Day NP, Verzilli C, Peacock SJ, , 2010. Defining the true sensitivity of culture for the diagnosis of melioidosis using Bayesian latent class models. PLoS One 5: e12485.[Crossref]
  25. Palasatien S, Lertsirivorakul R, Royros P, Wongratanacheewin S, Sermswan RW, , 2008. Soil physicochemical properties related to the presence of Burkholderia pseudomallei . Trans R Soc Trop Med Hyg 102 (Suppl 1): S5S9.[Crossref]
  26. Kaestli M, Mayo M, Harrington G, Ward L, Watt F, Hill JV, Cheng AC, Currie BJ, , 2009. Landscape changes influence the occurrence of the melioidosis bacterium Burkholderia pseudomallei in soil in northern Australia. PLoS Negl Trop Dis 3: e364.[Crossref]
  27. Wuthiekanun V, Anuntagool N, White NJ, Sirisinha S, , 2002. Short report: a rapid method for the differentiation of Burkholderia pseudomallei and Burkholderia thailandensis . Am J Trop Med Hyg 66: 759761.
  28. Trung TT, Hetzer A, Topfstedt E, Göhler A, Limmathurotsakul D, Wuthiekanun V, Peacock SJ, Steinmetz I, , 2011. Improved culture-based detection and quantification of Burkholderia pseudomallei from soil. Trans R Soc Trop Med Hyg 105: 346351.[Crossref]
  29. Chen YS, Chen SC, Kao CM, Chen YL, , 2003. Effects of soil pH, temperature and water content on the growth of Burkholderia pseudomallei . Folia Microbiol (Praha) 48: 253256.[Crossref]
  30. Herigstad B, Hamilton M, Heersink J, , 2001. How to optimize the drop plate method for enumerating bacteria. J Microbiol Methods 44: 121129.[Crossref]
  31. Thomas G, , 1996. Soil pH and soil acidity. Methods of Soil Analysis, Part 3: Chemical Methods. Madison, WI: Soil Science Society of America, 475490.
  32. Rhoades J, , 1996. Salinity: electrical conductivity and total dissolved solids. Methods of Soil Analysis, Part 3: Chemical Methods. Madison, WI: Soil Science Society of America, 417435.
  33. Jones J, , 1930. Laboratory Guide for Conducting Soil Tests and Plant Analysis. Boca Raton, FL: CRC Press.
  34. Walkley A, Black I, , 1934. An examination of the Degtjareff method for determining organic carbon in soils: effect of variations in digestion conditions and of inorganic soil constituents. Soil Sci 63: 251263.[Crossref]
  35. Hoben HJ, Somasegaran P, , 1982. Comparison of the pour, spread, and drop plate methods for enumeration of Rhizobium spp. in inoculants made from presterilized peat. Appl Environ Microbiol 44: 12461247.
  36. Inglis TJ, Sagripanti JL, , 2006. Environmental factors that affect the survival and persistence of Burkholderia pseudomallei . Appl Environ Microbiol 72: 68656875.[Crossref]
  37. Tong S, Yang S, Lu Z, He W, , 1996. Laboratory investigation of ecological factors influencing the environmental presence of Burkholderia pseudomallei . Microbiol Immunol 40: 451453.[Crossref]
  38. Dejsirilert S, Kondo E, Chiewsilp D, Kanai K, , 1991. Growth and survival of Pseudomonas pseudomallei in acidic environments. Jpn J Med Sci Biol 44: 6374.[Crossref]
  39. Department of Land Development, 1995. Document for the Seminar on “Help Isan Fight Saline Soil.” Bangkok, Thailand: Department of Land Development.
  40. Bernstein L, , 1974. Crop growth and salinity. Agron J 17: 3954.
  41. Clermont Dauphin C, Suwannang N, Grünberger O, Hammecker C, Maeght JL, , 2010. Yield of rice under water and soil salinity risks in farmers fields in northeast Thailand. Field Crop Res 118: 289296.[Crossref]
  42. Accoe F, Boeckx P, Busschaert J, Hofman G, Van Cleemput O, , 2004. Gross N transformation rates and net N mineralisation rates related to the C and N contents of soil organic matter fractions in grassland soils of different age. Soil Biol Biochem 36: 20752087.[Crossref]
  43. Draper AD, Mayo M, Harrington G, Karp D, Yinfoo D, Ward L, Haslem A, Currie BJ, Kaestli M, , 2010. Association of the melioidosis agent Burkholderia pseudomallei with water parameters in rural water supplies in Northern Australia. Appl Environ Microbiol 76: 53055307.[Crossref]
  44. Yang HM, Chaowagul W, Sokol PA, , 1991. Siderophore production by Pseudomonas pseudomallei . Infect Immun 59: 776780.
  45. Kaestli M, Mayo M, Harrington G, Watt F, Hill J, Gal D, Currie BJ, , 2007. Sensitive and specific molecular detection of Burkholderia pseudomallei, the causative agent of melioidosis, in the soil of tropical northern Australia. Appl Environ Microbiol 73: 68916897.[Crossref]

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  • Received : 31 Jul 2013
  • Accepted : 05 Dec 2013

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