1921
Volume 90, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Etiologic studies of diarrhea are limited by uneven diagnostic methods and frequent asymptomatic detection of enteropathogens. Polymerase chain reaction-based stool pathogen quantification may help distinguish clinically significant infections. We performed a nested case-control study of diarrhea in infants from a community-based birth cohort in Tanzania. We tested 71 diarrheal samples and pre-diarrheal matched controls with a laboratory-developed TaqMan Array Card for 19 enteropathogens. With qualitative detection, no pathogens were significantly associated with diarrhea. When pathogen quantity was considered, rotavirus (odds ratio [OR] = 2.70 per log increase, < 0.001), astrovirus (OR = 1.49, = 0.01), and /enteroinvasive (OR = 1.47, = 0.04) were associated with diarrhea. Enterotoxigenic (0.15 SD decline in length-for-age z score after 3 months per log increase, < 0.001) and / (0.11 SD decline, = 0.003) in pre-diarrheal stools were associated with poor linear growth. Quantitative analysis can help refine the association between enteropathogens and disease in endemic settings.

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2014-01-08
2017-11-19
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  • Received : 30 Jul 2013
  • Accepted : 14 Sep 2013

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