1921
Volume 89, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Leptospirosis is a widespread but under-reported cause of morbidity and mortality. Global re-emergence of leptospirosis has been associated with the growth of informal urban settlements in which rodents are thought to be important reservoir hosts. Understanding the multi-host epidemiology of leptospirosis is essential to control and prevent disease. A cross-sectional survey of rodents in the Kibera settlement in Nairobi, Kenya was conducted in September–October 2008 to demonstrate the presence of pathogenic leptospires. A real-time quantitative polymerase chain reaction showed that 41 (18.3%) of 224 rodents carried pathogenic leptospires in their kidneys, and sequence data identified and in this population. Rodents of the genus (37 of 185) were significantly more likely to be positive than those of the genus (4 of 39; odds ratio = 15.03). Questionnaire data showed frequent contact between humans and rodents in Kibera. This study emphasizes the need to quantify the public health impacts of this neglected disease at this and other urban sites in Africa.

[open-access] This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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2017-11-18
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References

  1. World Health Organization, 1999. Leptospirosis worldwide, 1999. Wkly Epidemiol Rec 74: 237242.
  2. Levett PN, , 2001. Leptospirosis. Clin Microbiol Rev 14: 296326.[Crossref]
  3. Hartskeerl RA, Collares-Pereira M, Ellis WA, , 2011. Emergence, control and re-emerging leptospirosis: dynamics of infection in the changing world. Clin Microbiol Infect 17: 494501.[Crossref]
  4. Biggs HM, Bui DM, Galloway RL, Stoddard RA, Shadomy SV, Morrissey AB, Bartlett JA, Onyango JJ, Maro VP, Kinabo GD, Saganda W, Crump JA, , 2011. Leptospirosis among hospitalized febrile patients in northern Tanzania. Am J Trop Med Hyg 85: 275281.[Crossref]
  5. Levett PN, , 2003. Usefulness of serologic analysis as a predictor of the infecting serovar in patients with severe leptospirosis. Clin Infect Dis 36: 447452.[Crossref]
  6. Machang'u RS, Mgode GF, Assenga J, Mhamphi G, Weetjens B, Cox C, Verhagen R, Sondij S, Goris MG, Hartskeerl RA, , 2004. Serological and molecular characterization of Leptospira serovar Kenya from captive African giant pouched rats Cricetomys gambianus from Morogoro Tanzania. FEMS Immunol Med Microbiol 41: 117121.[Crossref]
  7. Holt J, Davis S, Leirs H, , 2006. A model of leptospirosis infection in an African rodent to determine risk to humans: seasonal fluctuations and the impact of rodent control. Acta Trop 99: 218225.[Crossref]
  8. Mgode GF, Machang'u RS, Goris MG, Engelbert M, Sondij S, Hartskeerl RA, , 2006. New Leptospira serovar Sokoine of serogroup Icterohaemorrhagiae from cattle in Tanzania. Int J Syst Evol Microbiol 56: 593597.[Crossref]
  9. Matthias MA, Ricaldi JN, Cespedes M, Diaz MM, Galloway RL, Saito M, Steigerwalt AG, Patra KP, Ore CV, Gotuzzo E, Gilman RH, Levett PN, Vinetz JM, , 2008. Human leptospirosis caused by a new, antigenically unique Leptospira associated with a Rattus species reservoir in the Peruvian Amazon. PLoS Negl Trop Dis 2: e213.[Crossref]
  10. Reller ME, Bodinayake C, Nagahawatte A, Devasiri V, Kodikara-Arachichi W, Strouse JJ, Flom JE, Dumler JS, Woods CW, , 2011. Leptospirosis as frequent cause of acute febrile illness in southern Sri Lanka. Emerg Infect Dis 17: 16781684.[Crossref]
  11. Kendall EA, LaRocque RC, Bui DM, Galloway R, Ari MD, Goswami D, Breiman RF, Luby S, Brooks WA, , 2010. Leptospirosis as a cause of fever in urban Bangladesh. Am J Trop Med Hyg 82: 11271130.[Crossref]
  12. Ismail TF, Wasfy MO, Abdul-Rahman B, Murray CK, Hospenthal DR, Abdel-Fadeel M, Abdel-Maksoud M, Samir A, Hatem ME, Klena J, Pimentel G, El-Sayed N, Hajjeh R, , 2006. Retrospective serosurvey of leptospirosis among patients with acute febrile illness and hepatitis in Egypt. Am J Trop Med Hyg 75: 10851089.
  13. Feikin DR, Olack B, Bigogo GM, Audi A, Cosmas L, Aura B, Burke H, Njenga MK, Williamson J, Breiman RF, , 2011. The burden of common infectious disease syndromes at the clinic and household level from population-based surveillance in rural and urban Kenya. PLoS ONE 6: e16085.[Crossref]
  14. World Health Organization, 2004. Leptospirosis in Kenya. Available at: http://www.who.int/csr/don/2004_06_17a/en/. Accessed August 26, 2013.
  15. Ari MD, Guracha A, Fadeel MA, Njuguna C, Njenga MK, Kalani R, Abdi H, Warfu O, Omballa V, Tetteh C, Breiman RF, Pimentel G, Feikin DR, , 2011. Challenges of establishing the correct diagnosis of outbreaks of acute febrile illnesses in Africa: the case of a likely Brucella outbreak among nomadic pastoralists, northeast Kenya, March–July 2005. Am J Trop Med Hyg 85: 909912.[Crossref]
  16. de Geus A, Wolff JW, Timmer VE, , 1977. Clinical leptospirosis in Kenya (II): a field study in Nyanza Province. East Afr Med J 54: 125132.
  17. de Geus A, Wolff JW, Timmer VE, , 1977. Clinical leptospirosis in Kenya (1): a clinical study in Kwale District, Coast Province. East Afr Med J 54: 115124.
  18. Forrester AT, Kranendonk O, Turner LH, Wolff JW, Bohlander HJ, , 1969. Serological evidence of human leptospirosis in Kenya. East Afr Med J 46: 497506.
  19. Ball MG, , 1966. Animal hosts of leptospires in Kenya and Uganda. Am J Trop Med Hyg 15: 523530.
  20. Reis RB, Ribeiro GS, Felzemburgh RD, Santana FS, Mohr S, Melendez A, Queiroz A, Santos AC, Ravines RR, Tassinari WS, Carvalho MS, Reis MG, Ko AI, , 2008. Impact of environment and social gradient on Leptospira infection in urban slums. PLoS Negl Trop Dis 2: e228.[Crossref]
  21. Oliviera DS, Guimarães MJ, Portugal JL, Medeiros Z, , 2009. The socio-demographic, environmental and reservoir factors associated with leptospirosis in an urban area of north-eastern Brazil. Ann Trop Med Parasitol 103: 149157.[Crossref]
  22. Maciel EA, de Carvalho ALF, Nascimento SF, de Matos RB, Gouveia EL, Reis MG, Ko AI, , 2008. Household transmission of Leptospira infection in urban slum communities. PLoS Negl Trop Dis 2: e154.[Crossref]
  23. Sarkar U, Nascimento SF, Barbosa R, Martins R, Nuevo H, Kalafanos I, Grunstein I, Flannery B, Dias J, Riley LW, Reis MG, Ko AI, , 2002. Population-based case-control investigation of risk factors for leptospirosis during an urban epidemic. Am J Trop Med Hyg 66: 605610.
  24. Gulis G, Mulumba JA, Juma O, Kakosova B, , 2004. Health status of people of slums in Nairobi, Kenya. Environ Res 96: 219227.[Crossref]
  25. Mills JN, Childs JE, Ksiazek TG, Peters CJ, Velleca WM, , 1995. Methods for Trapping and Sampling Small Mammals for Virologic Testing. Available at: http://www.cdc.gov/hantavirus/resources/materials.html. Accessed August 16, 2013.
  26. QIAGEN, 2006. DNeasy Blood and Tissue Handbook. Available at: http://www.qiagen.com/Knowledge-and-Support/Resource-Center/Resource-Search/?q=DNeasy%3b&l=en%3b. Accessed August 26, 2013.
  27. Ahmed A, Engelberts MF, Boer KR, Ahmed N, Hartskeerl RA, , 2009. Development and validation of a real-time PCR for detection of pathogenic Leptospira species in clinical materials. PLoS ONE 4: e7093.[Crossref]
  28. Victoria B, Ahmed A, Zuerner RL, Ahmed N, Bulach DM, Quinteiro J, Hartskeerl RA, , 2008. Conservation of the S10-spc α locus within otherwise highly plastic genomes provides phylogenetic insight into the genus Leptospira . PLoS ONE 3: e2752.[Crossref]
  29. Houemenou G, Ahmed A, Libois R, Hartskeerl RA, , 2013. Leptospira spp. prevalence in small mammal populations in Cotonou, Benin. ISRN Epidemiol 2013.
  30. R Development Core Team, 2009. R: A Language and Environment for Statistical Computing. Available at: http://www.R-project.org. Accessed August 26, 2013.
  31. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, , 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 27312739.[Crossref]
  32. Saitou N, Nei M, , 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406425.
  33. Tamura K, Nei M, Kumar S, , 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 101: 1103011035.[Crossref]
  34. Ahmed N, Devi SM, Valverde Mdl A, Vijayachari P, Machang'u RS, Ellis WA, Hartskeerl RA, , 2006. Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species. Ann Clin Microbiol Antimicrob 5: 28.[Crossref]
  35. Ko AI, Reis MG, Dourado CMR, Johnson WD, Riley LW, , 1999. Urban epidemic of severe leptospirosis in Brazil. Salvador Leptospirosis Study Group. Lancet 354: 820825.[Crossref]
  36. Johnson MA, Smith H, Joseph P, Gilman RH, Bautista CT, Campos KJ, Cespedes M, Klatsky P, Vidal C, Terry H, Calderon MM, Coral C, Cabrera L, Parmar PS, Vinetz JM, , 2004. Environmental exposure and leptospirosis, Peru. Emerg Infect Dis 10: 10161022.[Crossref]
  37. Agudelo-Flórez P, Londoño AF, Quiroz VH, Angel JC, Moreno N, Loaiza ET, Muñoz LF, Rodas JD, , 2009. Prevalence of Leptospira spp. in urban rodents from a groceries trade center of Medellín, Colombia. Am J Trop Med Hyg 81: 906910.[Crossref]
  38. Benacer D, Zain SN, Amran F, Galloway RL, Thong KL, , 2013. Isolation and molecular characterization of Leptospira interrogans and Leptospira borgpetersenii isolates from the urban rat populations of Kuala Lumpur, Malaysia. Am J Trop Med Hyg 88: 704709.[Crossref]
  39. de Faria MT, Calderwood MS, Athanazio DA, McBride AJ, Hartskeerl RA, Pereira MM, Ko AI, Reis MG, , 2008. Carriage of Leptospira interrogans among domestic rats from an urban setting highly endemic for leptospirosis in Brazil. Acta Trop 108: 15.[Crossref]
  40. Easterbrook JD, Kaplan JB, Vanasco NB, Reeves WK, Purcell RH, Kosoy MY, Glass GE, Watson J, Klein SL, , 2007. A survey of zoonotic pathogens carried by Norway rats in Baltimore, Maryland, USA. Epidemiol Infect 135: 11921199.[Crossref]
  41. Halliday JEB, , 2010. Animal Sentinel Surveillance: Evaluating Domestic Dogs as Sentinels for Zoonotic Pathogen Surveillance. Available at: http://www.era.lib.ed.ac.uk/handle/1842/4794. Accessed August 26, 2013.
  42. Vinetz JM, , 2001. Leptospirosis. Curr Opin Infect Dis 14: 527538.[Crossref]
  43. Ganoza CA, Matthias MA, Collins-Richards D, Brouwer KC, Cunningham CB, Segura ER, Gilman RH, Gotuzzo E, Vinetz JM, , 2006. Determining risk for severe leptospirosis by molecular analysis of environmental surface waters for pathogenic Leptospira . PLoS Med 3: 13291340.[Crossref]
  44. Rahelinirina S, Leon A, Harstskeerl RA, Sertour N, Ahmed A, Raharimanana C, Ferquel E, Garnier M, Chartier L, Duplantier JM, Rahalison L, Cornet M, , 2010. First isolation and direct evidence for the existence of large small-mammal reservoirs of Leptospira sp. in Madagascar. PLoS ONE 5: e14111.[Crossref]
  45. Bunnell JE, Hice CL, Watts DM, Montrueil V, Tesh RB, Vinetz JM, , 2000. Detection of pathogenic Leptospira spp. infections among mammals captured in the Peruvian Amazon basin region. Am J Trop Med Hyg 63: 255258.
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  • Received : 18 Jul 2013
  • Accepted : 27 Aug 2013

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