1921
Volume 90, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Presently, global efforts are being made to control and eradicate the deadliest tropical diseases through the improvement of adequate interventions. A critical point for programs to succeed is the prompt and accurate diagnosis in endemic regions. Rapid diagnostic tests (RDTs) are being massively deployed and used to improve diagnosis in tropical countries. In the present report, we evaluated the hypothesis of, after use for diagnosis, the reuse of the RDT kit as a DNA source, which can be used downstream as a molecular surveillance and/or quality control tool. As a proof of principle, a polymerase chain reaction-based method was used to detect spp. minicircle kinetoplast DNA from leishmaniasis RDT kits. Our results show that spp. DNA can be extracted from used RDTs and may constitute an important, reliable, and affordable tool to assist in future leishmaniasis molecular surveillance methods.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.13-0329
2014-06-04
2017-09-24
Loading full text...

Full text loading...

/deliver/fulltext/14761645/90/6/1082.html?itemId=/content/journals/10.4269/ajtmh.13-0329&mimeType=html&fmt=ahah

References

  1. Desjeux P, , 2004. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27: 305318.[Crossref]
  2. Chappuis F, Sundar S, Hailu A, Ghalib H, Rijal S, Peeling RW, Alvar J, Boelaert M, , 2007. Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nat Rev Microbiol 5: 873882.[Crossref]
  3. Zijlstra EE, Ali MS, el-Hassan AM, el-Toum IA, Satti M, Ghalib HW, Kager PA, , 1992. Kala-azar: a comparative study of parasitological methods and the direct agglutination test in diagnosis. Trans R Soc Trop Med Hyg 86: 505507.[Crossref]
  4. Gatti S, Gramegna M, Klersy C, Madama S, Bruno A, Maserati R, Bernuzzi AM, Cevini C, Scaglia M, , 2004. Diagnosis of visceral leishmaniasis: the sensitivities and specificities of traditional methods and a nested PCR assay. Ann Trop Med Parasitol 98: 667676.[Crossref]
  5. Antinori S, Calattini S, Longhi E, Bestetti G, Piolini R, Magni C, Orlando G, Gramiccia M, Acquaviva V, Foschi A, Corvasce S, Colomba C, Titone L, Parravicini C, Cascio A, Corbellino M, , 2007. Clinical use of polymerase chain reaction performed on peripheral blood and bone marrow samples for the diagnosis and monitoring of visceral leishmaniasis in HIV-infected and HIV-uninfected patients: a single-center, 8-year experience in Italy and review of the literature. Clin Infect Dis 44: 16021610.[Crossref]
  6. Pandey K, Pant S, Kanbara H, Shuaibu MN, Mallik AK, Pandey BD, Kaneko O, Yanagi T, , 2008. Molecular detection of Leishmania parasites from whole bodies of sandflies collected in Nepal. Parasitol Res 103: 293297.[Crossref]
  7. Aransay AM, Scoulica E, Tselentis Y, , 2000. Detection and identification of Leishmania DNA within naturally infected sand flies by seminested PCR on minicircle kinetoplastic DNA. Appl Environ Microbiol 66: 19331938.[Crossref]
  8. Srivastava P, Mehrotra S, Tiwary P, Chakravarty J, Sundar S, , 2011. Diagnosis of Indian visceral leishmaniasis by nucleic acid detection using PCR. PLoS One 6: e19304.[Crossref]
  9. Chargui N, Haouas N, Jaouadi K, Gorcii M, Pratlong F, Dedet JP, Mezhoud H, Babba H, , 2012. Usefulness of a PCR-based method in the detection and species identification of Leishmania from clinical samples. Pathol Biol (Paris) 60: e75e79.[Crossref]
  10. Salam MA, Khan MG, Bhaskar KR, Afrad MH, Huda MM, Mondal D, , 2012. Peripheral blood buffy coat smear: a promising tool for diagnosis of visceral leishmaniasis. J Clin Microbiol 50: 837840.[Crossref]
  11. Kent RJ, Norris DE, , 2005. Identification of mammalian blood meals in mosquitoes by a multiplexed polymerase chain reaction targeting cytochrome B. Am J Trop Med Hyg 73: 336342.
  12. Edgar RC, , 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 17921797.[Crossref]
  13. Brandonisio O, Fumarola L, Maggi P, Cavaliere R, Spinelli R, Pastore G, , 2002. Evaluation of a rapid immunochromatographic test for serodiagnosis of visceral leishmaniasis. Eur J Clin Microbiol Infect Dis 21: 461464.[Crossref]
  14. Sundar S, Pai K, Sahu M, Kumar V, Murray HW, , 2002. Immunochromatographic strip-test detection of anti-K39 antibody in Indian visceral leishmaniasis. Ann Trop Med Parasitol 96: 1923.[Crossref]
  15. Carvalho SF, Lemos EM, Corey R, Dietze R, , 2003. Performance of recombinant K39 antigen in the diagnosis of Brazilian visceral leishmaniasis. Am J Trop Med Hyg 68: 321324.
  16. Bern C, Jha SN, Joshi AB, Thakur GD, Bista MB, , 2000. Use of the recombinant K39 dipstick test and the direct agglutination test in a setting endemic for visceral leishmaniasis in Nepal. Am J Trop Med Hyg 63: 153157.
  17. Sarker CB, Momen A, Jamal MF, Siddiqui NI, Siddiqui FM, Chowdhury KS, Rahman S, Talukder SI, , 2003. Immunochromatographic (rK39) strip test in the diagnosis of visceral leishmaniasis in Bangladesh. Mymensingh Med J 12: 9397.
  18. Zijlstra EE, Nur Y, Desjeux P, Khalil EA, El-Hassan AM, Groen J, , 2001. Diagnosing visceral leishmaniasis with the recombinant K39 strip test: experience from the Sudan. Trop Med Int Health 6: 108113.[Crossref]
  19. Boelaert M, Rijal S, Regmi S, Singh R, Karki B, Jacquet D, Chappuis F, Campino L, Desjeux P, Le Ray D, Koirala S, Van der Stuyft P, , 2004. A comparative study of the effectiveness of diagnostic tests for visceral leishmaniasis. Am J Trop Med Hyg 70: 7277.
  20. Sundar S, Reed SG, Singh VP, Kumar PC, Murray HW, , 1998. Rapid accurate field diagnosis of Indian visceral leishmaniasis. Lancet 351: 563565.[Crossref]
  21. WHO, 2011. Visceral Leishmaniasis Rapid Diagnostic Test Performance. Geneva: World Health Organization.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.13-0329
Loading
/content/journals/10.4269/ajtmh.13-0329
Loading

Data & Media loading...

Supplementary Data

Supplementary PDF

  • Received : 05 Jun 2013
  • Accepted : 23 Jan 2014

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error