Volume 89, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Laboratory-reared were exposed to soil and wild-caught feces for 1 week. Fleas from these two treatments and a control group of laboratory-reared fleas were infected with , the etiological agent of plague. Fleas exposed to soil transmitted to mice at a significantly greater rate (50.0% of mice were infected) than control fleas (23.3% of mice were infected). Although the concentration of in fleas did not differ among treatments, the minimum transmission efficiency of fleas from the soil and wild flea feces treatments (6.9% and 7.6%, respectively) were more than three times higher than in control fleas (2.2%). Our results suggest that exposing laboratory-reared fleas to diverse microbes alters transmission of .


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  1. Chandler JA, Lang JM, Bhatnagar S, Eisen JA, Kopp A, , 2011. Bacterial communities of diverse Drosophila species: ecological context of a host-microbe model system. PLoS Genet 7: e1002272.[Crossref] [Google Scholar]
  2. Jones RT, Vetter SM, Montenieiri J, Holmes J, Bernhardt SA, Gage KL, , 2013. Yersinia pestis infection and laboratory conditions alter flea-associated bacterial communities. ISME J 7: 224228.[Crossref] [Google Scholar]
  3. Lehman RM, Lundgren JG, Petzke LM, , 2009. Bacterial communities associated with the digestive tract of the predatory ground beetle, Poecilus chalcites, and their modification by laboratory rearing and antibiotic treatment. Microb Ecol 57: 349358.[Crossref] [Google Scholar]
  4. Tsiropoulos GJ, , 1983. Microflora associated with wild and laboratory reared adult olive fruit flies, Dacus-oleae (GMEL). Z Ang Entomol-J Appl Entomol 96: 337340.[Crossref] [Google Scholar]
  5. Wang H, Jin L, Zhang H, , 2011. Comparison of the diversity of the bacterial communities in the intestinal tract of adult Bactrocera dorsalis from three different populations. J Appl Microbiol 110: 13901401.[Crossref] [Google Scholar]
  6. Azambuja P, Garcia ES, Ratcliffe NA, , 2005. Gut microbiota and parasite transmission by insect vectors. Trends Parasitol 21: 568572.[Crossref] [Google Scholar]
  7. Weiss B, Aksoy S, , 2011. Microbiome influences on insect host vector competence. Trends Parasitol 27: 514522.[Crossref] [Google Scholar]
  8. Craven RB, Maupin GO, Beard ML, Quan TJ, Barnes AM, , 1993. Reported cases of human plague infections in the United States, 1970–1991. J Med Entomol 30: 758761.[Crossref] [Google Scholar]
  9. Eisen RJ, Eisen L, Gage KL, , 2009. Studies of vector competency and efficiency of North American fleas for Yersinia pestis: state of the field and future research needs. J Med Entomol 46: 737744.[Crossref] [Google Scholar]
  10. Engelthaler DM, Hinnebusch BJ, Rittner CM, Gage KL, , 2000. Quantitative competitive PCR as a technique for exploring flea - Yersinia pestis dynamics. Am J Trop Med Hyg 62: 552560. [Google Scholar]
  11. Eisen RJ, Bearden SW, Wilder AP, Montenieri JA, Antolin MF, Gage KL, , 2006. Early-phase transmission of Yersinia pestis by unblocked fleas as a mechanism explaining rapidly spreading plague epizootics. Proc Natl Acad Sci USA 103: 1538015385.[Crossref] [Google Scholar]
  12. Biggerstaff B, , 2006. PooledInfRate: a Microsoft Excel Add-In to Compute Prevalence Estimates from Pooled Samples. Fort Collins, CO: Centers for Disease Control and Prevention. [Google Scholar]
  13. Eisen RJ, Borchert JN, Holmes JL, Amatre G, Van Wyk K, Enscore RE, Babi N, Atiku LA, Wilder AP, Vetter SM, Bearden SW, Montenieri JA, Gage KL, , 2008. Early-phase transmission of Yersinia pestis by cat fleas (Ctenocephalides felis) and their potential role as vectors in a plague-endemic region of Uganda. Am J Trop Med Hyg 78: 949956. [Google Scholar]
  14. Eisen RJ, Holmes JL, Schotthoefer AM, Vetter SM, Montenieri JA, Gage KL, , 2008. Demonstration of early-phase transmission of Yersinia pestis by the mouse flea, Aetheca wagneri (Siphonaptera: Ceratophylidae), and implications for the role of deer mice as enzootic reservoirs. J Med Entomol 45: 11601164.[Crossref] [Google Scholar]
  15. Eisen RJ, Lowell JL, Montenieri JA, Bearden SW, Gage KL, , 2007. Temporal dynamics of early-phase transmission of Yersinia pestis by unblocked fleas: secondary infectious feeds prolong efficient transmission by Oropsylla montana (Siphonaptera: Ceratophyllidae). J Med Entomol 44: 672677.[Crossref] [Google Scholar]
  16. Eisen RJ, Wilder AP, Bearden SW, Montenieri JA, Gage KL, , 2007. Early-phase transmission of Yersinia pestis by unblocked Xenopsylla cheopis (Siphonaptera: Pulicidae) is as efficient as transmission by blocked fleas. J Med Entomol 44: 678682.[Crossref] [Google Scholar]
  17. Wilder AP, Eisen RJ, Bearden SW, Montenieri JA, Gage KL, Antolin MF, , 2008. Oropsylla hirsuta (Siphonaptera: Ceratophyllidae) can support plague epizootics in black-tailed prairie dogs (Cynomys ludovicianus) by early-phase transmission of Yersinia pestis . Vector Borne Zoonotic Dis 8: 359367.[Crossref] [Google Scholar]
  18. Wilder AP, Eisen RJ, Bearden SW, Montenieri JA, Tripp DW, Brinkerhoff RJ, Gage KL, Antolin MF, , 2008. Transmission efficiency of two flea species (Oropsylla tuberculata cynomuris and Oropsylla hirsuta) involved in plague epizootics among prairie dogs. EcoHealth 5: 205212.[Crossref] [Google Scholar]
  19. Douglas JR, Wheeler CM, , 1941. Sylvatic plague studies. II. The fate of Pasteurelia pestis in the flea. J Infect Dis 72: 1830.[Crossref] [Google Scholar]
  20. Eskey CR, Haas VH, , 1940. Plague in Rodents and Fleas in the Western Part of the United States. Public Health Bulletin: 83. [Google Scholar]
  21. Lorange EA, Race BL, Sebbane F, Hinnebusch BJ, , 2005. Poor vector competence of fleas and the evolution of hypervirulence in Yersinia pestis . J Infect Dis 191: 19071912.[Crossref] [Google Scholar]
  22. Wheeler CM, Douglas JR, , 1945. Sylvatic plague studies. 5. The determination of vector efficiency. J Infect Dis 77: 112.[Crossref] [Google Scholar]

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  • Received : 15 Mar 2013
  • Accepted : 13 May 2013
  • Published online : 09 Oct 2013

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