Volume 89, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Canine African trypanosomosis (CAT) is rarely reported in the literature. In this preliminary study, we evaluated the performance of loop-mediated isothermal amplification (LAMP) against microscopy to detect CAT in six exotic dog breeds naturally infected with trypanosomes from Zambia's South Luangwa National Park and Chiawa Game Management Area. To our knowledge, this is the first report of CAT in Zambia. The patients exhibited a variety of aspecific clinical signs. The LAMP did not only confirm all six parasitologically positive CAT cases detected passively between April 2010 and January 2012, but was also critical in trypanosome speciation. According to LAMP, the majority of the dogs had monolytic infections with either or . The LAMP is thus a potential simple and cost-effective tool for trypanosome diagnosis in endemic regions. The rare report of zoonotic trypanosomes in dogs in Zambia has public health implications and justifies further investigations of CAT.


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  1. Dantas-Torres F, , 2008. Canine vector-borne diseases in Brazil. Parasit Vectors 1: 25.[Crossref] [Google Scholar]
  2. Matete GO, , 2003. Occurrence, clinical manifestation and the epidemiological implications of naturally occurring canine trypanosomosis in western Kenya. Onderstepoort J Vet Res 70: 317323.[Crossref] [Google Scholar]
  3. Gow AG, Simpson JW, Picozzi K, , 2007. The first report of canine African trypanosomosis in the UK. J Small Anim Pract 48: 658661.[Crossref] [Google Scholar]
  4. Laohasinnarong D, Thekisoe OM, Malele I, Namangala B, Ishii A, Goto Y, Kawazu SI, Sugimoto C, Inoue N, , 2011. Prevalence of Trypanosoma sp. in cattle from Tanzania estimated by conventional PCR and loop-mediated isothermal amplification (LAMP). Parasitol Res 109: 17351739.[Crossref] [Google Scholar]
  5. Keck N, Herder S, Kaba D, Solano P, Gomez J, Cuny G, Davoust B, , 2009. Epidemiological study of canine trypanosomosis in an urban area of Ivory Coast. Parasite 16: 305308.[Crossref] [Google Scholar]
  6. Namangala B, Hachaambwa L, Kajino K, Mweene AS, Hayashida K, Simuunza M, Simukoko H, Choongo K, Chansa P, Lakhi S, Moonga L, Chota A, Ndebe J, Nsakashalo-Senkwe M, Chizema E, Kasonka L, Sugimoto C, , 2012. The use of loop-mediated isothermal amplification (LAMP) to detect the re-emerging Human African Trypanosomiasis (HAT) in the Luangwa and Zambezi Valleys. Parasit Vectors 5: 282.[Crossref] [Google Scholar]
  7. Thekisoe OM, Kuboki N, Nambota A, Fujisaki K, Sugimoto C, Igarashi I, Yasuda J, Inoue N, , 2007. Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis. Acta Trop 102: 182189.[Crossref] [Google Scholar]
  8. Njiru ZK, Mikosza AS, Armstrong T, Enyaru JC, Ndung'u JM, Thompson AR, , 2008b. Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense . PLoS Negl Trop Dis 2: e147.[Crossref] [Google Scholar]
  9. Njiru ZK, Mikosza AS, Matovu E, Enyaru JC, Ouma JO, Kibona SN, Thompson RC, Ndung'u JM, , 2008a. African trypanosomiasis: sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA. Int J Parasitol 38: 589599.[Crossref] [Google Scholar]
  10. Horchner F, Zillmann U, Metzner M, Schonefeld A, Hehlitz D, , 1985. West African dogs as a model for research on trypanotolerance. Trop Med Parasitol 36: 257258. [Google Scholar]
  11. Matovu E, Kuepfer I, Boobo A, Kibona S, Burri C, , 2010. Comparative detection of trypanosomal DNA by loop-mediated isothermal amplification and PCR from flinders technology associates cards spotted with patient blood. J Clin Microbiol 48: 20872090.[Crossref] [Google Scholar]

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  • Received : 09 Feb 2013
  • Accepted : 18 Apr 2013
  • Published online : 10 Jul 2013

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