1921
Volume 89, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of -specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance of these recombinant antigens was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive sera from febrile patients in Peru, 22 sera were indigenous MAT-negative febrile patient sera, and 36 sera were from patients with other febrile diseases from Southeast Asia, where leptospirosis is also endemic. Combining the results of immunoglobulin M (IgM) and IgG detection from these three antigens, the overall sensitivity is close to 90% based on the MAT. These results suggest that an ELISA using multiple recombinant antigens may be used as an alternative method for the detection of -specific antibodies.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.13-0041
2013-12-04
2017-11-17
Loading full text...

Full text loading...

/deliver/fulltext/14761645/89/6/1088.html?itemId=/content/journals/10.4269/ajtmh.13-0041&mimeType=html&fmt=ahah

References

  1. Pappas G, Papadimitriou P, Siozopoulou V, Christou L, Akritidis N, , 2008. The globalization of leptospirosis: worldwide incidence trends. Int J Infect Dis 12: 351357.[Crossref]
  2. Levett PN, , 2001. Leptospirosis. Clin Microbiol Rev 14: 296326.[Crossref]
  3. Hartskeerl RA, , 2012. Leptospira. Molecular Detection of Human Bacterial Pathogens. Boca Raton, FL: CRC Press Taylor and Francis Group, 11691188.
  4. Cruz LS, Vargas R, Lopes AA, , 2009. Leptospirosis: a worldwide resurgent zoonosis and important cause of acute renal failure and death in developing nations. Ethn Dis 19: 3741.
  5. Sethi S, Sharma N, Kakkar N, Taneja J, Chatterjee SS, Banga SS, Sharma M, , 2010. Increasing trends of leptospirosis in northern India: a clinico-epidemiological study. PLoS Negl Trop Dis 4: 579.[Crossref]
  6. Victoriano AF, Smythe LD, Gloriani-Barzaga N, Cavinta LL, Kasai T, Limpakarnjanarat K, Ong BL, Gongal G, Hall J, Coulombe CA, Yanagihara Y, Yoshida S, Adler B, , 2009. Leptospirosis in the Asia Pacific region. BMC Infect Dis 9: 147.[Crossref]
  7. Lim VK, , 2011. Leptospirosis: a re-emerging infection. Malays J Pathol 33: 15.
  8. Reller ME, Bodinayake C, Nagahawatte A, Devasiri V, Kodikara-Arachichi W, Strouse JJ, Flom JE, Dumler JS, Woods CW, , 2011. Leptospirosis as frequent cause of acute febrile illness in southern Sri Lanka. Emerg Infect Dis 17: 16781684.[Crossref]
  9. Bourhy P, Collet L, Lernout T, Zinini F, Hartskeerl RA, van der Linden H, Thiberge JM, Diancourt L, Brisse S, Giry C, Pettinelli F, Picardeau M, , 2012. Human leptospira isolates circulating in Mayotte (Indian Ocean) have unique serological and molecular features. J Clin Microbiol 50: 307311.[Crossref]
  10. Fonzar UJ, Langoni H, , 2012. Geographic analysis on the occurrence of human and canine leptospirosis in the city of Maringá, state of Paraná, Brazil. Rev Soc Bras Med Trop 45: 100105.[Crossref]
  11. Lau CL, Dobson AJ, Smythe LD, Fearnley EJ, Skelly C, Clements AC, Craig SB, Fuimaono SD, Weinstein P, , 2012. Leptospirosis in American Samoa 2010: epidemiology, environmental drivers, and the management of emergence. Am J Trop Med Hyg 86: 309319.[Crossref]
  12. Zhang C, Wang H, Yan J, , 2012. Leptospirosis prevalence in Chinese populations in the last two decades. Microbes Infect 14: 317323.[Crossref]
  13. Vinetz JM, Glass GE, Flexner CE, Mueller P, Kaslow DC, , 1996. Sporadic urban leptospirosis. Ann Intern Med 125: 794798.[Crossref]
  14. Sejvar J, Bancroft E, Winthrop K, Bettinger J, Bajani M, Bragg S, Kaiser R, Marano N, Popovic T, Tappero J, Ashford D, Mascola L, Vugia D, Perkins B, Rosenstein N, , 2003. Leptospirosis in “Eco-Challenge” atheletes, Malaysian Borneo, 2000. Emerg Infect Dis 9: 702707.[Crossref]
  15. Morgan J, Borstein SL, Karpati AM, Bruce M, Bolin CA, Austin CC, Woods CW, Lingappa J, Langkop C, Davis B, Graham DR, Proctor M, Ashford DA, Bajani M, Bragg SL, Shutt K, Perkins BA, Tappero JW, , 2002. Outbreak of leptospirosis among triathlon participants and community residents in Springfield, Illinois, 1998. Clin Infect Dis 34: 15931599.[Crossref]
  16. Centers for Disease Control and Prevention, 1997. Outbreak of leptospirosis among white-water rafters—Costa Rica, 1996. MMWR Morb Mortal Wkly Rep 46: 577579.
  17. Boland M, Sayers G, Cileman T, Bergin C, Sheehan N, Creamer E, O'Connell M, Jones L, Zochowski W, , 2004. A cluster of leptospirosis cases in canoeists following a competition on the River Liffey. Epidemiol Infect 132: 195200.[Crossref]
  18. Radl C, Müller M, Revilla-Fernandez S, Karner-Zuser S, de Martin A, Schauer U, Karner F, Stanek G, Balcke P, Hallas A, Frank H, Fürnschlief A, Erhart F, Allerberger F, , 2011. Outbreak of leptospirosis among triathlon participants in Langau, Austria, 2010. Wien Klin Wochenschr 123: 751755.[Crossref]
  19. Kouadio IK, Aljunid S, Kamigaki T, Hammad K, Oshitani H, , 2012. Infectious diseases following natural disasters: prevention and control measures. Expert Rev Anti Infect Ther 10: 95104.[Crossref]
  20. Amilasan AS, Ujiie M, Suzuki M, Salva E, Belo MC, Koizumi N, Yoshimatsu K, Schmidt WP, Marte S, Dimaano EM, Villarama JB, Ariyoshi K, , 2012. Outbreak of leptospirosis after flood, the Philippines, 2009. Emerg Infect Dis 18: 9194.[Crossref]
  21. Lin CY, Chiu NC, Lee CM, , 2012. Leptospirosis after typhoon. Am J Trop Med Hyg 86: 187188.[Crossref]
  22. Smith JK, Young MM, Wilson KL, Craig SB, , 2012. Leptospirosis following a major flood in Central Queensland, Australia. Epidemiol Infect 25: 16.
  23. Faine SB, Adler B, Bolin C, Perolat P, , 1999. Leptospira and Leptospirosis, 2nd ed. Melbourne, Australia: MediSci.
  24. WHO, 2003. Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control. Malta: World Health Organization.
  25. Levett PN, , 2007. Leptospira. Manual of Clinical Microbiology, 9th ed. Washington, DC: American Society for Microbiology Press, 963970.
  26. Faine S, , 1982. Guidelines for the Control of Leptospirosis. Geneva: World Health Organization.
  27. Cumberland PC, Everard COR, Levett PN, , 1999. Assessment of the efficacy of the IgM enzyme-linked immunosorbent assay (ELISA) and microscopic agglutination test (MAT) in the diagnosis of acute leptospirosis. Am J Trop Med Hyg 61: 731734.
  28. Smits HL, Eapen CK, Sugathan S, Kuriakose M, Gasem MH, Yersin C, Sasaki D, Pujianto B, Vestering M, Abdoel TH, Gussenhoven GC, , 2001. Lateral-flow assay for rapid serodiagnosis of human leptospirosis. Clin Diagn Lab Immunol 8: 166169.
  29. Effler PV, Bogard AK, Domen HY, Katz AR, Higa HY, Sasaki DM, , 2002. Evaluation of eight rapid screening tests for acute leptospirosis in Hawaii. J Clin Microbiol 40: 14641469.[Crossref]
  30. Levett PN, Branch SL, , 2002. Evaluation of two enzyme-linked immunosorbent assay methods for detection of immunoglobulin M antibodies in acute leptospirosis. Am J Trop Med Hyg 66: 745748.
  31. Vijayachari P, Sugunan AP, Sehgal SC, , 2002. Evaluation of Lepto Dri Dot as a rapid test for the diagnosis of leptospirosis. Epidemiol Infect 129: 617621.[Crossref]
  32. Bajani MD, Ashford DA, Bragg SL, Woods CW, Aye T, Spiegel RA, Plikaytis BD, Perkins BA, Phelan M, Levett PN, Weyant RS, , 2003. Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis. J Clin Microbiol 41: 803809.[Crossref]
  33. Sehgal SC, Vijayachari P, Sugunan AP, Umapathi T, , 2003. Field application of Lepto lateral flow for rapid diagnosis of leptospirosis. J Med Microbiol 52: 897901.[Crossref]
  34. McBride AJ, Athanazio DA, Reis MG, Ko AI, , 2005. Leptospirosis. Curr Opin Infect Dis 18: 376386.[Crossref]
  35. Blacksell SD, Smythe L, Phetsouvanh R, Dohnt M, Hartskeerl R, Symonds M, Slack A, Vongsouvath M, Davong V, Lattana O, Phongmany S, Keoluangkot V, White NJ, Day NP, Newton PN, , 2006. Limited diagnostic capacity of two commercial assays for the detection of leptospira immunoglobulin M antibodies in Laos. Clin Vaccine Immunol 3: 11661169.[Crossref]
  36. Desakorn V, Wuthiekanun V, Thanachartwet V, Sahassananda D, Chierakul W, Apiwattanaporn A, Day NP, Limmathurotsakul D, Peacock SJ, , 2012. Accuracy of a commercial IgM ELISA for the diagnosis of human leptospirosis in Thailand. Am J Trop Med Hyg 86: 524527.[Crossref]
  37. Ren SX, Fu G, Jiang XG, Zeng R, Miao YG, Xu H, Zhang YX, Xiong H, Lu G, Lu LF, Jiang HQ, Jia J, Tu YF, Jiang JX, Gu WY, Zhang YQ, Cai Z, Sheng HH, Yin HF, Zhang Y, Zhu GF, Wan M, Huang HL, Qian Z, Wang SY, Ma W, Yao ZJ, Shen Y, Qiang BQ, Xia QC, Guo XK, Danchin A, Saint Girons I, Somerville RL, Wen YM, Shi MH, Chen Z, Xu JG, Zhao GP, , 2003. Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing. Nature 422: 888893.[Crossref]
  38. Nascimento AL, Ko AI, Martins EA, Monteiro-Vitorello CB, Ho PL, Haake DA, Verjovski-Almeida S, Hartskeerl RA, Marques MV, Oliveira MC, Menck CF, Leite LC, Carrer H, Coutinho LL, Degrave WM, Dellagostin OA, El-Dorry H, Ferro ES, Ferro MI, Furlan LR, Gamberini M, Giglioti EA, Góes-Neto A, Goldman GH, Goldman MH, Harakava R, Jerônimo SM, Junqueira-de-Azevedo IL, Kimura ET, Kuramae EE, Lemos EG, Lemos MV, Marino CL, Nunes LR, de Oliveira RC, Pereira GG, Reis MS, Schriefer A, Siqueira WJ, Sommer P, Tsai SM, Simpson AJ, Ferro JA, Camargo LE, Kitajima JP, Setubal JC, Van Sluys MA, , 2004. Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis. J Bacteriol 186: 21642172.[Crossref]
  39. Bulach DM, Zuerner RL, Wilson P, Seemann T, McGrath A, Cullen PA, Davis J, Johnson M, Kuczek E, Alt DP, Peterson-Burch B, Coppel RL, Rood JI, Davies JK, Adler B, , 2006. Genome reduction in Leptospira borgpetersenii reflects limited transmission potential. Proc Natl Acad Sci USA 103: 1456014565.[Crossref]
  40. Picardeau M, Bulach DM, Bouchier C, Zuerner RL, Zidane N, Wilson PJ, Creno S, Kuczek ES, Bommezzadri S, Davis JC, McGrath A, Johnson MJ, Boursaux-Eude C, Seemann T, Rouy Z, Coppel RL, Rood JI, Lajus A, Davies JK, Médigue C, Adler B, , 2008. Genome sequence of the saprophyte leptospira biflexa provided insights into the evolution of Leptospira and the pathogenesis of leptospirosis. PLoS One 3: e1607.[Crossref]
  41. Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM, , 2003. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3: 757771.[Crossref]
  42. Haake DA, Chao G, Zuerner RL, Barnett JK, Barnett D, Mazel M, Matsunaga J, Levett PN, Bolin CA, , 2000. The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection. Infect Immun 68: 22762285.[Crossref]
  43. Shang ES, Summers TA, Haake DA, , 1996. Molecular cloning and sequence analysis of the gene coding LipL41, a surface-exposed lipoprotein of pathogenic Leptospira species. Infect Immun 64: 23222330.
  44. Matsunaga J, Barocchi MA, Croda J, Young TA, Sanchez Y, Siqueira I, Bolin CA, Reis MG, Riley LW, Haake DA, Ko AI, , 2003. Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily. Mol Microbiol 49: 929945.[Crossref]
  45. Cullen PA, Xu X, Matsunaga J, Sanchez Y, Ko AI, Haake DA, Adler B, , 2005. Surfaceome of Leptospira spp. Infect Immun 73: 48534863.[Crossref]
  46. Mcbride AJ, Cerqueira GM, Suchard MA, Moreira AN, Zuerner RL, Reis MG, Haake DA, Ko AI, Dellagostin OA, , 2009. Genetic diversity of the leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp. Infect Genet Evol 9: 196205.[Crossref]
  47. Palaniappan RU, Chang YF, Jusuf SS, Artiushin S, Timoney JF, McDonough SP, Barr SC, Divers TJ, Simpson KW, McDonough PL, Mohammed HO, , 2002. Cloning and molecular characterization of an immunogenic LigA protein of Leptospira interrogans . Infect Immun 70: 59245930.[Crossref]
  48. Doungchawee G, Kositanont U, Niwetpathomwat A, Inwisai T, Sagarasaeranee P, Haake DA, , 2008. Early diadnosis of leptospirosis by immunoglobulin M immunoblot testing. Clin Vaccine Immunol 15: 492498.[Crossref]
  49. Studier FW, Moffatt BA, , 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189: 113130.[Crossref]
  50. Koizumi N, Watanabe H, , 2004. Leptospiral immunoglobulin-like proteins elicit protective immunity. Vaccine 22: 15451552.[Crossref]
  51. Chappel RJ, Goris M, Palmer MF, Hartskeerl RA, , 2004. Impact of proficiency testing on results of the microscopic agglutination test for diagnosis of leptospirosis. J Clin Microbiol 42: 54845488.[Crossref]
  52. Guerreiro H, Croda J, Flannery B, Mazel M, Reis MG, Levett PN, Ko AI, Haake DA, , 2001. Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans. Infect Immun 69: 49584968.[Crossref]
  53. Flannery B, Costa D, Carvalho FP, Guerreiro H, Matsunaga J, DaSilva ED, Ferreira AGP, Riley LW, Reis MG, Haake DA, Ko AI, , 2001. Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis. J Clin Microbiol 39: 33033310.[Crossref]
  54. Chapman AJ, Adler B, Faine S, , 1988. Antigens recognized by the human immune response to infection with Leptospira interrogans serovar hardjo. J Med Microbiol 25: 269278.[Crossref]
  55. Chapman AJ, Everard CO, Faine S, Adler B, , 1991. Antigens recognized by human immune response to severe leptospirosis in Barbados. Epidemiol Infect 107: 143155.[Crossref]
  56. Adler B, Murphy AM, Locarnini SA, Faine S, , 1980. Detection of specific antileptospiral immunoglobulins M and G in human serum by solid-phase enzyme-linked immunosorbent assay. J Clin Microbiol 11: 452457.
  57. Terpstra WJ, Ligthart GS, Schoone GJ, , 1985. ELISA for the detection of specific IgM and IgG in human leptospirosis. J Gen Microbiol 131: 377385.
  58. Engstrom SM, Shoop E, Johnson RC, , 1995. Immunoblot interpretation criteria for serodiagnosis of early lyme disease. J Clin Microbiol 55: 419427.
  59. Magnarelli LA, Ijdo JW, Padula SJ, Flavelli RA, Fikrig E, , 2000. Serologic diagnosis of Lyme borreliosis by using enzyme-linked immunosorbent assays with recombinant antigens. J Clin Microbiol 38: 17351739.
  60. Schmidt BL, Edjlalipour M, Luger A, , 2000. Comparative evaluation of nine different enzyme-linked immunosorbent assays for determination of antibodies against Treponema pallidum in patients with primary syphilis. J Clin Microbiol 38: 12791282.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.13-0041
Loading
/content/journals/10.4269/ajtmh.13-0041
Loading

Data & Media loading...

  • Received : 22 Jan 2013
  • Accepted : 14 Sep 2013

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error