Volume 89, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one -specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human species with a three-step PCR.

[open-access] This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


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  • Received : 16 Jan 2013
  • Accepted : 03 May 2013
  • Published online : 09 Oct 2013

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