Volume 89, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We collected anophelines every second week for one year from randomly selected houses in southwestern Ethiopia by using Centers for Disease Control (CDC) light traps, pyrethrum spray catches, and artificial pit shelter constructions to detect circumsporozoite proteins and estimate entomologic inoculation rates (EIRs). Of 3,678 tested for circumsporozoite proteins, 11 were positive for and three for . The estimated annual EIR of was 17.1 infectious bites per person per year (95% confidence interval = 7.03–34.6) based on CDC light traps and 0.1 infectious bites per person per year based on pyrethrum spray catches. The EIRs from CDC light traps varied from 0 infectious bites per person per year (in 60% of houses) to 73.2 infectious bites per person per year in the house nearest the breeding sites. Risk of exposure to infectious bites was higher in wet months than dry months, with a peak in April (9.6 infectious bites per person per month), the period of highest mosquito density.

[open-access] This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


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  1. Otten M, Aregawi M, Were W, Karema C, Medin A, Bekele W, Jima D, Gausi K, Komatsu R, Korenromp E, Low-Beer D, Grabowsky M, , 2009. Initial evidence of reduction of malaria cases and deaths in Rwanda and Ethiopia due to rapid scale-up of malaria prevention and treatment. Malar J 8: 14.[Crossref] [Google Scholar]
  2. Abose T, Yeebiyo Y, Olana D, Alamirew D, Beyene Y, Regassa L, Mengesha A, , 1998. Re-orientation and Definition of the Role of Malaria Vector Control in Ethiopia. Geneva: World Health Organization. [Google Scholar]
  3. Kelly-Hope L, McKenzie FE, , 2009. The multiplicity of malaria transmission: a review of entomological inoculation rate measurements and methods across sub-Saharan Africa. Malar J 8: 19.[Crossref] [Google Scholar]
  4. Shaukat A, Breman JG, McKenzie FE, , 2010. Using entomological inoculation rate to assess the impact of vector control on malaria parasite transmission and elimination. Malar J 9: 122.[Crossref] [Google Scholar]
  5. Coluzzi M, , 1984. Heterogeneities of the malaria vectorial system in tropical Africa and their significance in malaria epidemiology and control. Bull World Health Organ 62: 107113. [Google Scholar]
  6. Day J, , 2005. Host-seeking strategies of mosquito diseases vectors. J Am Mosq Control Assoc 21: 1722.[Crossref] [Google Scholar]
  7. Garrett-Jones C, Boreham PF, Pant CP, , 1980. Feeding habits of anophelines (Diptera: Culicidae) in 1971–1978, with reference to the human blood index: a review. Bull Entomol Res 70: 165185.[Crossref] [Google Scholar]
  8. Ulrich J, Naranjo DP, Alimi TO, Müller GC, Beier JC, , 2013. How much vector control is needed to achieve malaria elimination? Trends Parasitol 29: 104109.[Crossref] [Google Scholar]
  9. Hay S, Rogers DJ, Toomer JF, Snow RW, , 2000. Annual Plasmodium falciparum entomological inoculation rates (EIR) across Africa: literature survey, internet access and review. Trans R Soc Trop Med Hyg 94: 113127.[Crossref] [Google Scholar]
  10. Omumbo J, Guerrab CA, Hay SI, Snow RW, , 2005. The influence of urbanisation on measures of Plasmodium falciparum infection prevalence in east Africa. Acta Trop 93: 1121.[Crossref] [Google Scholar]
  11. Trape J, Rogier C, , 1996. Combating malaria morbidity and mortality by reducing transmission. Parasitol Today 12: 236240.[Crossref] [Google Scholar]
  12. World Health Organization, 1975. Manual on Practical Entomology in Malaria. Part 2: Methods and Techniques. Geneva: World Health Organization. [Google Scholar]
  13. Lines J, Curtis CF, Wilkes TJ, Njunwa T, , 1991. Monitoring human biting mosquitoes in Tanzania with light traps hung beside mosquito nets. Bull Entomol Res 81: 7784.[Crossref] [Google Scholar]
  14. Taye A, Hadis M, Adugna N, Tilahun D, Wirtz RA, , 2006. Biting behaviour and Plasmodium infection rates of Anopheles arabiensis from Sille, Ethiopia. Acta Trop 97: 5054.[Crossref] [Google Scholar]
  15. Krafsur E, , 1977. The bionomics and relative prevalence of Anopheles species with respect to the transmission of Plasmodium to man in western Ethiopia. J Med Entomol 25: 180194.[Crossref] [Google Scholar]
  16. Animut A, Gebre-Michael T, Balkew M, Lindtjørn B, , 2013. Blood meal sources and entomological inoculation rates of anophelines along a highland altitudinal transect in south-central Ethiopia. Malar J 12: 76.[Crossref] [Google Scholar]
  17. Loha E, Lindtjorn B, , 2012. Predictors of Plasmodium falciparum malaria incidence in Chano Mille, south Ethiopia: a longitudinal study. Am J Trop Med Hyg 87: 450459.[Crossref] [Google Scholar]
  18. Massebo F, Balkew M, Gebre-Michael T, Lindtjørn B, , 2013. Blood meal origins and insecticide susceptibility of Anopheles arabiensis from Chano in south-west Ethiopia. Parasit Vectors 6: 44.[Crossref] [Google Scholar]
  19. Viste E, Korecha D, Sorteberg A, , 2013. Recent drought and precipitation tendencies in Ethiopia. Theor Appl Climatol 112: 535551.[Crossref] [Google Scholar]
  20. Silver J, , 2008. Mosquito Ecology: Field Sampling Methods. New York: Springer.[Crossref] [Google Scholar]
  21. Gillies M, Coetzee M, , 1987. A supplement to the anopheline of Africa south of the Sahara. S Afr Inst Med Res 55: 143. [Google Scholar]
  22. Detinova T, , 1962. Age Grouping Methods in Diptera of Medical Importance, with Special Reference to Some Vectors of Malaria. Geneva: World Health Organization, Monograph Series, No. 47. [Google Scholar]
  23. Beier J, Perkins PV, Wirtz RA, Whitmire RE, Mugambi M, Hockmeyer WT, , 1987. Field evaluation of an enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum sporozoite detection in anopheline mosquitoes from Kenya. Am J Trop Med Hyg 36: 459468. [Google Scholar]
  24. Drakeley C, Schellenberg D, Kihondaj J, Sousa CA, Arez AP, Lopes D, Lines J, Mshinda IH, Lengelers C, Schellenberg AJ, Tanner M, Alonso P, , 2003. An estimation of the entomological inoculation rate for Ifakara: a semi-urban area in a region of intense malaria transmission. Trop Med Int Health 8: 767774.[Crossref] [Google Scholar]
  25. World Health Organization, 2003. Malaria Entomology and Vector Control. Geneva: World Health Organization. [Google Scholar]
  26. Garrett-Jones C, , 1964. The human blood index of malaria vectors in relation to epidemiological assessment. Bull World Health Organ 30: 241261. [Google Scholar]
  27. Ndiath M, Mazenot C, Gaye A, Konate L, Bouganali C, Faye O, Sokhna C, Trape JF, , 2011. Methods to collect Anopheles mosquitoes and evaluate malaria transmission: a comparative study in two villages in Senegal. Malar J 10: 270.[Crossref] [Google Scholar]
  28. Fornadel C, Norris LC, Norris DE, , 2010. Centers for disease control light traps for monitoring Anopheles arabiensis human biting rates in an area with low vector density and high insecticide-treated bed net use. Am J Trop Med Hyg 83: 838842.[Crossref] [Google Scholar]
  29. Overgaard H, Sæbø S, Reddy MR, Reddy VP, Abaga S, Matias A, Slotman MA, , 2012. Light traps fail to estimate reliable malaria mosquito biting rates on Bioko Island, Equatorial Guinea. Malar J 11: 56.[Crossref] [Google Scholar]
  30. Nigatu W, Petros B, Lulu M, Adugna N, Wirtz R, , 1994. Species composition, feeding and resting behaviour of the common anthropophilic anopheline mosquitoes in relation to malaria transmission in Gambella, south-west Ethiopia. Int J Trop Insect Sci 15: 371377.[Crossref] [Google Scholar]
  31. Ameneshewa B, Service MW, , 1996. The relationship between female body size and survival rate of the malaria vector Anopheles arabiensis in Ethiopia. Med Vet Entomol 10: 170172.[Crossref] [Google Scholar]
  32. Charlwood J, Smith T, Kihonda J, Heiz B, Billingsley PF, Takken W, , 1995. Density independent feeding success of malaria vectors (Diptera: Culicidae) in Tanzania. Bull Entomol Res 85: 2935.[Crossref] [Google Scholar]
  33. Tirados IC, Gibson G, Torr SJ, , 2006. Blood feeding behaviour of the malaria mosquito Anopheles arabiensis: implications for vector control. Med Vet Entomol 20: 425437.[Crossref] [Google Scholar]
  34. Loha E, Lunde TM, Lindtjørn B, , 2012. Effect of bednets and indoor residual spraying on spatio-temporal clustering of malaria in a village in south Ethiopia: a longitudinal study. PLoS ONE 7: e47354.[Crossref] [Google Scholar]
  35. Ribeiro J, Seulu F, Abose T, Kidane G, Teklehaimanot A, , 1996. Temporal and spatial distribution of anopheline mosquitoes in an Ethiopian villages: implications for malaria control strategies. Bull World Health Organ 74: 299305. [Google Scholar]
  36. Kibret S, Alemu Y, Boelee E, Tekie H, Alemu D, Petros B, , 2010. The impact of a small-scale irrigation scheme on malaria transmission in Ziway area, central Ethiopia. Trop Med Int Health 15: 4150. [Google Scholar]
  37. Shililu J, Ghebremeskel T, Mengistu S, Fekadu H, Zerom M, Mbogo C, Githure J, Novak R, Brantly E, Beier JC, , 2003. High seasonal variation in entomologic inoculation rates in Eritrea, a semi-arid region of unstable malaria in Africa. Am J Trop Med Hyg 69: 607613. [Google Scholar]
  38. Smith D, Dushoff J, Snow RW, Hay SI, , 2005. The entomological inoculation rate and Plasmodium falciparum infection in African children. Nature 438: 492495.[Crossref] [Google Scholar]
  39. Himeidan Y, Elzaki MM, Kweka EJ, Ibrahim M, Elhassan IM, , 2011. Pattern of malaria transmission along the Rahad River basin, eastern Sudan. Parasit Vectors 4: 109.[Crossref] [Google Scholar]
  40. Molineaux L, Gramiccia G, , 1980. The Garki Project: Research on the Epidemiology and Control of Malaria in the Sudan Savanna of West Africa. Geneva: World Health Organization. [Google Scholar]
  41. Beier J, Killeen GF, Githure JI, , 1999. Short report: entomologic inoculation rates and Plasmodium falciparum malaria prevalence in Africa. Am J Trop Med Hyg 61: 109113. [Google Scholar]

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  • Received : 12 Dec 2012
  • Accepted : 14 Jun 2013
  • Published online : 04 Sep 2013

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