1921
Volume 88, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Toxoplasmosis in humans and other animals is caused by the protozoan parasite . During the process of host cell invasion and parasitophorous vacuole formation by the tachyzoites, the parasite secretes Rhoptry protein 8 (), an apical secretory organelle. Thus, is an important protein for the pathogenesis of . The DNA was constructed into a pVAX-1 vaccine vector and used for immunizing BALB/c mice. Immunized mice developed immune response characterized by significant antibody responses, antigen-specific proliferation of spleen cells, and production of high levels of IFN-γ (816 ± 26.3 pg/mL). Challenge experiments showed significant levels of increase in the survival period (29 days compared with 9 days in control) in DNA vaccinated mice after a lethal challenge with . Results presented in this study suggest that DNA is a promising and potential vaccine candidate against toxoplasmosis.

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2013-05-01
2017-09-22
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  • Received : 04 Dec 2012
  • Accepted : 28 Jan 2013

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