1921
Volume 88, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Toxoplasmosis in humans and other animals is caused by the protozoan parasite . During the process of host cell invasion and parasitophorous vacuole formation by the tachyzoites, the parasite secretes Rhoptry protein 8 (), an apical secretory organelle. Thus, is an important protein for the pathogenesis of . The DNA was constructed into a pVAX-1 vaccine vector and used for immunizing BALB/c mice. Immunized mice developed immune response characterized by significant antibody responses, antigen-specific proliferation of spleen cells, and production of high levels of IFN-γ (816 ± 26.3 pg/mL). Challenge experiments showed significant levels of increase in the survival period (29 days compared with 9 days in control) in DNA vaccinated mice after a lethal challenge with . Results presented in this study suggest that DNA is a promising and potential vaccine candidate against toxoplasmosis.

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References

  1. Dubey JP, 2008. The history of Toxoplasma gondii–the first 100 years. J Eukaryot Microbiol 55: 467475.[Crossref]
    [Google Scholar]
  2. Fatoohi A, Cozon G, Greenland T, Ferrandiz J, Bienvenu J, Picot S, Peyron F, 2002. Cellular immune responses to recombinant antigens in pregnant women chronically infected with Toxoplasma gondii . Clin Diagn Lab Immunol 9: 704707.
    [Google Scholar]
  3. McLeod R, Kieffer F, Sautter M, Hosten T, Pelloux H, 2009. Why prevent, diagnose and treat congenital toxoplasmosis? Mem Inst Oswaldo Cruz 104: 320344.[Crossref]
    [Google Scholar]
  4. Yuan ZG, Zhang XX, He XH, Petersen E, Zhou DH, He Y, Lin RQ, Li XZ, Chen XL, Shi XR, 2011. Protective immunity induced by Toxoplasma gondii rhoptry protein 16 against toxoplasmosis in mice. Clin Vaccine Immunol 18: 119124.[Crossref]
    [Google Scholar]
  5. Hill D, Dubey J, 2002. Toxoplasma gondii: transmission, diagnosis and prevention. Clin Microbiol Infect 8: 634640.[Crossref]
    [Google Scholar]
  6. Bhopale GM, 2003. Development of a vaccine for toxoplasmosis: current status. Microbes Infect 5: 457462.[Crossref]
    [Google Scholar]
  7. Yuan ZG, Zhang XX, Lin RQ, Petersen E, He S, Yu M, He XH, Zhou DH, He Y, Li HX, 2011. Protective effect against toxoplasmosis in mice induced by DNA immunization with gene encoding Toxoplasma gondii ROP18. Vaccine 29: 66146619.[Crossref]
    [Google Scholar]
  8. Buxton D, 1993. Toxoplasmosis: the first commercial vaccine. Parasitol Today 9: 335337.[Crossref]
    [Google Scholar]
  9. Buxton D, Innes E, 1995. A commercial vaccine for ovine toxoplasmosis. Parasitology-Cambridge 110: 11.[Crossref]
    [Google Scholar]
  10. Jongert E, Roberts CW, Gargano N, Förster-Waldl E, Petersen E, 2009. Vaccines against Toxoplasma gondii: challenges and opportunities. Mem Inst Oswaldo Cruz 104: 252266.[Crossref]
    [Google Scholar]
  11. Ulmer JB, Donnelly JJ, Parker SE, Rhodes GH, Felgner PL, Dwarki V, Gromkowski SH, Deck RR, Dewitt CM, Friedman A, 1993. Heterologous protection against influenza by injection of DNA encoding a viral protein. Science 259: 17451749.[Crossref]
    [Google Scholar]
  12. Robinson H, 1999. DNA vaccines: basic mechanism and immune responses (Review). Int J Mol Med 4: 549550.
    [Google Scholar]
  13. Spencer JA, Smith BF, Guarino AJ, Blagburn BL, Baker HJ, 2004. The use of CpG as an adjuvant to Toxoplasma gondii vaccination. Parasitol Res 92: 313316.[Crossref]
    [Google Scholar]
  14. Denkers EY, Gazzinelli RT, 1998. Regulation and function of T-cell-mediated immunity during Toxoplasma gondii infection. Clin Microbiol Rev 11: 569588.
    [Google Scholar]
  15. Angus C, Klivington-Evans D, Dubey J, Kovacs JA, 2000. Immunization with a DNA plasmid encoding the SAG1 (P30) protein of Toxoplasma gondii is immunogenic and protective in rodents. J Infect Dis 181: 317324.[Crossref]
    [Google Scholar]
  16. Jongert E, De Craeye S, Dewit J, Huygen K, 2007. GRA7 provides protective immunity in cocktail DNA vaccines against Toxoplasma gondii . Parasite Immunol 29: 445453.[Crossref]
    [Google Scholar]
  17. Carey KL, Jongco AM, Kim K, Ward GE, 2004. The Toxoplasma gondii rhoptry protein ROP4 is secreted into the parasitophorous vacuole and becomes phosphorylated in infected cells. Eukaryot Cell 3: 13201330.[Crossref]
    [Google Scholar]
  18. Beckers C, Dubremetz JF, Mercereau-Puijalon O, Joiner KA, 1994. The Toxoplasma gondii rhoptry protein ROP 2 is inserted into the parasitophorous vacuole membrane, surrounding the intracellular parasite, and is exposed to the host cell cytoplasm. J Cell Biol 127: 947961.[Crossref]
    [Google Scholar]
  19. Hanks SK, Hunter T, 1995. Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification. FASEB J 9: 576596.
    [Google Scholar]
  20. Sinai AP, Joiner KA, 2001. The Toxoplasma gondii protein ROP2 mediates host organelle association with the parasitophorous vacuole membrane. J Cell Biol 154: 95108.[Crossref]
    [Google Scholar]
  21. Qiu W, Wernimont A, Tang K, Taylor S, Lunin V, Schapira M, Fentress S, Hui R, Sibley LD, 2009. Novel structural and regulatory features of rhoptry secretory kinases in Toxoplasma gondii . EMBO J 28: 969979.[Crossref]
    [Google Scholar]
  22. Ismael AB, Sekkai D, Collin C, Bout D, Mévélec MN, 2003. The MIC3 gene of Toxoplasma gondii is a novel potent vaccine candidate against toxoplasmosis. Infect Immun 71: 62226228.[Crossref]
    [Google Scholar]
  23. Gazzinelli R, Hakim F, Hieny S, Shearer G, Sher A, 1991. Synergistic role of CD4+ and CD8+ T lymphocytes in IFN-gamma production and protective immunity induced by an attenuated Toxoplasma gondii vaccine. J Immunol 146: 286.
    [Google Scholar]
  24. Jordan KA, Hunter CA, 2010. Regulation of CD8+ T cell responses to infection with parasitic protozoa. Exp Parasitol 126: 318325.[Crossref]
    [Google Scholar]
  25. Mévélec MN, Bout D, Desolme B, Marchand H, Magné R, Bruneel O, Buzoni-Gatel D, 2005. Evaluation of protective effect of DNA vaccination with genes encoding antigens GRA4 and SAG1 associated with GM-CSF plasmid, against acute, chronical and congenital toxoplasmosis in mice. Vaccine 23: 44894499.[Crossref]
    [Google Scholar]
  26. Desolme B, Mévélec MN, Buzoni-Gatel D, Bout D, 2000. Induction of protective immunity against toxoplasmosis in mice by DNA immunization with a plasmid encoding Toxoplasma gondii GRA4 gene. Vaccine 18: 25122521.[Crossref]
    [Google Scholar]
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  • Received : 04 Dec 2012
  • Accepted : 28 Jan 2013
  • Published online : 01 May 2013
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