Volume 88, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The Laboratory Without Walls is a modular field application of molecular biology that provides clinical laboratory support in resource-limited, remote locations. The current repertoire arose from early attempts to deliver clinical pathology and public health investigative services in remote parts of tropical Australia, to address the shortcomings of conventional methods when faced with emerging infectious diseases. Advances in equipment platforms and reagent chemistry have enabling rapid progress, but also ensure the Laboratory Without Walls is subject to continual improvement. Although new molecular biology methods may lead to more easily deployable clinical laboratory capability, logistic and technical governance issues continue to act as important constraints on wider implementation.


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  1. Inglis TJ, Garrow SC, Adams C, Henderson M, Mayo M, , 1998. Dry-season outbreak of melioidosis in Western Australia. Lancet 352: 1600.[Crossref] [Google Scholar]
  2. Rolim DB, Vilar DC, Sousa AQ, Miralles IS, de Oliveira DC, Harnett G, O'Reilly L, Howard K, Sampson I, Inglis TJ, , 2005. Melioidosis, northeastern Brazil. Emerg Infect Dis 11: 14581460.[Crossref] [Google Scholar]
  3. Inglis TJ, Levy A, Merritt AJ, Hodge M, McDonald R, Woods DE, , 2009. Melioidosis risk in a tropical industrial environment. Am J Trop Med Hyg 80: 7884. [Google Scholar]
  4. Inglis TJ, Merritt A, Montgomery J, Jayasinghe I, Thevanesam V, McInnes R, , 2008. Deployable laboratory response to emergence of melioidosis in central Sri Lanka. J Clin Microbiol 46: 34793481.[Crossref] [Google Scholar]
  5. Inglis TJ, Merritt A, Jayasinghe I, Montgomery J, Thevanesam V, , 2008. Logistic aspects of a deployable molecular microbiology laboratory. J Mil Vet Health 17: 610. [Google Scholar]
  6. Inglis TJ, Murray RJ, Watson M, , 2009. Trouble in paradise – Tropical, Emergency and Disaster Medicine Conference and Tropical Medicine Summit, Broome, Western Australia, 22–24 May 2009. Med J Aust 191: 597598. [Google Scholar]
  7. Inglis TJ, Merritt AJ, Levy A, Vietheer P, Bradbury R, Scholler A, Chidlow G, Smith DW, , 2011. Deployable laboratory response to influenza pandemic: PCR assay field trials and comparison with reference methods. PLoS ONE 6: e25526.[Crossref] [Google Scholar]
  8. Brumpton BM, McPherson BA, Frances SP, Inglis TJ, McCall B, , 2011. Townsville Field training area health assessment. ADF Health Journal 12: 4550. [Google Scholar]
  9. Merritt AJ, Keehner T, O'Reilly LC, McInnes RL, Inglis TJ, , 2010. Multiplex-amplified nominal tandem repeat analysis (MANTRA), a rapid method for genotyping Mycobacterium tuberculosis by use of multiplex PCR and a microfluidic laboratory chip. J Clin Microbiol 48: 37583761.[Crossref] [Google Scholar]
  10. 2012. Lab Without Walls expedition reports on the worldwide web. Available at: http://labwithoutwalls.org/projects. Accessed January 2013.
  11. Kunakorn M, Markham RB, , 1995. Clinically practical seminested PCR for Burkholderia pseudomallei quantitated by enzyme immunoassay with and without solution hybridization. J Clin Microbiol 33: 21312135. [Google Scholar]
  12. Pearson JE, Edwards S, , 2006. Transportation of reagents, reference materials and samples: the international perspective. Dev Biol (Basel) 126: 6170. [Google Scholar]
  13. Agampodi SB, Peacock SJ, Thevanesam V, Nugegoda DB, Smythe L, Thaipadungpanit J, Craig SB, Burns MA, Dohnt M, Boonsilp S, Senaratne T, Kumara A, Palihawadana P, Perera S, Vinetz JM, , 2011. Leptospirosis outbreak in Sri Lanka in 2008: lessons for assessing the global burden of disease. Am J Trop Med Hyg 85: 471478.[Crossref] [Google Scholar]
  14. De Silva N, Wijesundara S, Liyanapathirana V, Thevanesam V, Stenos J, , 2012. Scrub typhus among pediatric patients in Dambadeniya: a base hospital in Sri Lanka. Am J Trop Med Hyg 87: 342344.[Crossref] [Google Scholar]
  15. Cameron PA, Rainer TH, de Villiers Smit P, , 2003. The SARS epidemic: lessons for Australia. Med J Aust 178: 478479. [Google Scholar]
  16. Isaacs D, Dwyer DE, Hampson AW, , 2004. Avian influenza and planning for pandemics. Med J Aust 181: 6263. [Google Scholar]
  17. Hanna JN, Ritchie SA, Richards AR, Humphreys JL, Montgomery BL, Ehlers GJ, Pyke AT, Taylor CT, , 2009. Dengue in north Queensland, 2005–2008. Commun Dis Intell 33: 198203. [Google Scholar]
  18. Currie AJ, Heslop DJ, Winter SM, , 2011. H1N1 in the field: the impact on Australian Defence Force Field exercise Talisman Sabre 09. Australas Emerg Nurs J 14: 103107.[Crossref] [Google Scholar]
  19. 2012. Oxford Dictionary online definition of logistics. Available at: http://oxforddictionaries.com/definition/logistics?q=logistics. Accessed December 2012.
  20. Park S, Zhang Y, Lin S, Wang TH, Yang S, , 2011. Advances in microfluidic PCR for point-of-care infectious disease diagnostics. Biotechnol Adv 29: 830839.[Crossref] [Google Scholar]
  21. Wallet F, Nseir S, Bauman L, Herwegh S, Sendid B, Boulo M, Roussel-Delvallez M, Durocher AV, Courcol RJ, , 2010. Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood. Clin Microbiol Infect 16: 774779.[Crossref] [Google Scholar]
  22. Parida M, Horioke K, Ishida H, Dash PK, Saxena P, Jana AM, Islam MA, Inoue S, Hosaka N, Morita K, , 2005. Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription loop-mediated isothermal amplification assay. J Clin Microbiol 43: 28952903.[Crossref] [Google Scholar]
  23. Poschl B, Waneesorn J, Thekisoe O, Chitipongvivate S, Karanis P, , 2010. Comparative diagnosis of malaria infections by microscopy, nested PCR, and LAMP I northern Thailand. Am J Trop Med Hyg 83: 5660.[Crossref] [Google Scholar]
  24. Nagdev KV, Kashyap RS, Parida MM, Kapgate RC, Purohit HJ, Taori GM, Daginawala HF, , 2011. Loop-mediated isothermal amplification for rapid and reliable diagnosis of tuberculosis meningitis. J Clin Microbiol 49: 18611865.[Crossref] [Google Scholar]

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  • Received : 24 Nov 2012
  • Accepted : 09 Jan 2013
  • Published online : 03 Apr 2013

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