1921
Volume 88, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

accounts for an increasing fraction of malaria infections in Thailand and Cambodia. We compared genetic complexity and antimalarial resistance patterns in the two countries. Use of a heteroduplex tracking assay targeting the merozoite surface protein 1 gene revealed that vivax infections in both countries are frequently polyclonal (84%), with parasites that are highly diverse ( = 0.86) but closely related ( = 0.18). Following a history of different drug policies in Thailand and Cambodia, distinct patterns of antimalarial resistance have emerged: most Cambodian isolates harbor the multidrug resistance gene 1 () 976F mutation associated with chloroquine resistance (89% versus 8%, < 0.001), whereas Thai isolates more often display increased copy number (39% versus 4%, < 0.001). Finally, genotyping of paired isolates from individuals suspected of suffering relapse supports a complex scheme of relapse whereby recurrence of multiple identical variants is sometimes accompanied by the appearance of novel variants.

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Supplementary PDF

  • Received : 22 Nov 2012
  • Accepted : 28 Jan 2013
  • Published online : 05 Jun 2013

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