1921
Volume 89, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Rickettsioses caused by are an emergent global threat. Historically, the northern region of the province of Caldas in Colombia has reported murine typhus cases, and recently, serological studies confirmed high seroprevalence for both and . In the present study, fleas from seven municipalities were collected from dogs, cats, and mice. DNA was extracted and amplified by polymerase chain reaction (PCR) to identify , , and genes. Positive samples were sequenced to identify the species of . Of 1,341 fleas, was the most prevalent (76.7%). Positive PCR results in the three genes were evidenced in (minimum infection rates; 5.3%), (9.2%), and (10.0%). Basic Local Alignment Search Tool (BLAST) analyses of sequences showed high identity values (> 98%) with , and all were highly related by phylogenetic analyses. This work shows the first detection of in fleas collected from animals in Colombia.

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  • Received : 20 Nov 2012
  • Accepted : 24 May 2013

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