1921
Volume 89, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The aim of this translational study was to show the use of molecular surveillance for polymorphisms and copy number as a monitoring tool to track the emergence and dynamics of drug resistance. A molecular baseline for Suriname was established in 2005, with chloroquine resistance transporter () and multidrug resistance () markers and copy number in 40 samples. The baseline results revealed the existence of a uniformly distributed mutated genotype corresponding with the fully mefloquine-sensitive 7G8-like genotype (Y184F, S1034C, N1042D, and D1246Y) and a fixed N86 haplotype. All samples harbored the pivotal K76T mutation, showing that chloroquine reintroduction should not yet be contemplated in Suriname. After 5 years, 40 samples were assessed to trace temporal changes in the status of polymorphisms and copy number and showed minor genetic alterations in the gene and no significant changes in copy number, thus providing scientific support for prolongation of the current drug policy in Suriname.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.12-0695
2013-08-07
2019-06-26
Loading full text...

Full text loading...

/deliver/fulltext/14761645/89/2/311.html?itemId=/content/journals/10.4269/ajtmh.12-0695&mimeType=html&fmt=ahah

References

  1. Fidock DA, Nomura T, Talley AK, Cooper RA, Dzekunov SM, Ferdig MT, Ursos LM, Sidhu AB, Naudé B, Deitsch KW, Su XY, Wootton JC, Roepe PD, Wellems TE, , 2000. Mutations in the P. falciparum digestive vacuole transmembrane protein PfCRT and evidence for their role in chloroquine resistance. Mol Cell 6: 861.[Crossref] [Google Scholar]
  2. Plowe CV, Cortese JF, Djimde A, Nwanyanwu OC, Watkins WM, Winstanley PA, Estrada-Franco JG, Mollinedo RE, Avila JC, Cespedes JL, Carter D, Doumbo OK, , 1997. Mutations in Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase and epidemiologic patterns of pyrimethamine-sulfadoxine use and resistance. J Infect Dis 176: 15901596.[Crossref] [Google Scholar]
  3. Foote SJ, Galatis D, Cowman AF, , 1990. Amino acids in the dihydrofolate reductase-thymidylate synthase gene of Plasmodium falciparum involved in cycloguanil resistance differ from those involved in pyrimethamine resistance. Proc Natl Acad Sci USA 87: 3014.[Crossref] [Google Scholar]
  4. Srivastava IK, Morrisey JM, Darrouzet E, Daldal F, Vaidya AB, , 1999. Resistance mutations reveal the atovaquone-binding domain of cytochrome b in malaria parasites. Mol Microbiol 33: 704711.[Crossref] [Google Scholar]
  5. Wellems TE, Plowe CV, , 2001. Chloroquine-resistant malaria. J Infect Dis 184: 770776.[Crossref] [Google Scholar]
  6. Chen N, Russell B, Staley J, Kotecka B, Nasveld P, Cheng Q, , 2001. Sequence polymorphisms in pfcrt are strongly associated with chloroquine resistance in Plasmodium falciparum . J Infect Dis 183: 15431545.[Crossref] [Google Scholar]
  7. Reed MB, Saliba KJ, Caruana SR, Kirk K, Cowman AF, , 2000. Pgh1 modulates sensitivity and resistance to multiple antimalarials in Plasmodium falciparum . Nature 403: 906909.[Crossref] [Google Scholar]
  8. Duraisingh MT, Cowman AF, , 2005. Contribution of the pfmdr1 gene to antimalarial drug-resistance. Acta Trop 94: 181190.[Crossref] [Google Scholar]
  9. Pickard AL, Wongsrichanalai C, Purfield A, Kamwendo D, Emery K, Zalewski C, Kawamoto F, Miller RS, Meshnick SR, , 2003. Resistance to antimalarials in Southeast Asia and genetic polymorphisms in pfmdr1. Antimicrob Agents Chemother 47: 24182423.[Crossref] [Google Scholar]
  10. Anderson TJC, Nair S, Qin H, Singlam S, Brockman A, Paiphun L, Nosten F, , 2005. Are transporter genes other than the chloroquine resistance locus (pfcrt) and multidrug resistance gene (pfmdr) associated with antimalarial drug resistance? Antimicrob Agents Chemother 49: 21802188.[Crossref] [Google Scholar]
  11. Price RN, Cassar C, Brockman A, Duraisingh M, van Vugt M, White NJ, Nosten F, Krishna S, , 1999. The pfmdr1 gene is associated with a multidrug-resistant phenotype in Plasmodium falciparum from the western border of Thailand. Antimicrob Agents Chemother 43: 2943. [Google Scholar]
  12. Price RN, Uhlemann AC, Brockman A, McGready R, Ashley E, Phaipun L, Patel R, Laing K, Looareesuwan S, White NJ, Nosten F, Krishna S, , 2004. Mefloquine resistance in Plasmodium falciparum and increased pfmdr1 gene copy number. Lancet 364: 438447.[Crossref] [Google Scholar]
  13. Sidhu ABS, Uhlemann A, Valderramos SG, Valderramos J, Krishna S, Fidock DA, , 2006. Decreasing pfmdr1 copy number in Plasmodium falciparum malaria heightens susceptibility to mefloquine, lumefantrine, halofantrine, quinine, and artemisinin. J Infect Dis 194: 528535.[Crossref] [Google Scholar]
  14. Nelson AL, Purfield A, Mcdaniel P, Uthaimongkol N, Buathong N, Sriwichai S, Miller RS, Wongsrichanalai C, Meshnick SR, , 2005. pfmdr1 genotyping and in vivo mefloquine resistance on the Thai-Myanmar border. Am J Trop Med Hyg 72: 586592. [Google Scholar]
  15. Price RN, Uhlemann AC, van Vugt M, Brockman A, Hutagalung R, Nair S, Nash D, Singhasivanon P, Anderson TJC, Krishna S, White NJ, Nosten F, , 2006. Molecular and pharmacological determinants of the therapeutic response to artemether-lumefantrine in multidrug-resistant Plasmodium falciparum malaria. Clin Infect Dis 42: 15701577.[Crossref] [Google Scholar]
  16. Oostburg BF, , 1973. Chloroquin-resistant tropical malaria in South Suriname. Ned Tijdschr Geneeskd 117: 693694. [Google Scholar]
  17. Kublin JG, Cortese JF, Njunju EM, Mukadam RA, Wirima JJ, Kazembe PN, Djimdé AA, Kouriba B, Taylor TE, Plowe CV, , 2003. Reemergence of chloroquine-sensitive Plasmodium falciparum malaria after cessation of chloroquine use in Malawi. J Infect Dis 187: 18701875.[Crossref] [Google Scholar]
  18. Calvosa VS, Adagu IS, Póvoa MM, , 2001. Plasmodium falciparum: emerging mefloquine resistance in vitro in Para State, north Brazil. Trans R Soc Trop Med Hyg 95: 330331.[Crossref] [Google Scholar]
  19. Dondorp AM, Nosten F, Yi P, Das D, Phyo AP, Tarning J, Lwin KM, Ariey F, Hanpithakpong W, Lee SJ, Ringwald P, Silamut K, Imwong M, Chotivanich K, Lim P, Herdman T, An SS, Yeung S, Singhasivanon P, Day NPJ, Lindegardh N, Socheat D, White NJ, , 2009. Artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med 361: 455467.[Crossref] [Google Scholar]
  20. ABS, 2005. Census Office 7th General Population and Housing Census in Suriname. Suriname in Cijfers (no.213-2005/02), Algemeen Bureau voor de Statistiek, Paramaribo. [Google Scholar]
  21. Fischer A, Lejczak C, Lambert C, Servais J, Makombe N, Rusine J, Staub T, Hemmer R, Schneider F, Schmit JC, Arendt V, , 2004. Simple DNA extraction method for dried blood spots and comparison of two PCR assays for diagnosis of vertical human immunodeficiency virus type 1 transmission in Rwanda. J Clin Microbiol 42: 1620.[Crossref] [Google Scholar]
  22. Djimdé A, Doumbo OK, Cortese JF, Kayentao K, Doumbo S, Diourté Y, Coulibaly D, Dicko A, Su X, Nomura T, Fidock DA, Wellems TE, Plowe CV, , 2001. A molecular marker for chloroquine-resistant falciparum malaria. N Engl J Med 344: 257263.[Crossref] [Google Scholar]
  23. Plummer WB, Pereira LMP, Carrington CV, , 2004. Pfcrt and pfmdr1 alleles associated with chloroquine resistance in Plasmodium falciparum from Guyana, South America. Mem Inst Oswaldo Cruz 99: 389392.[Crossref] [Google Scholar]
  24. Zalis MG, Pang L, Silveira MS, Milhous WK, Wirth DF, , 1998. Characterization of Plasmodium falciparum isolated from the Amazon region of Brazil: evidence for quinine resistance. Am J Trop Med Hyg 58: 630637. [Google Scholar]
  25. Liu DQ, Liu RJ, Ren DX, Gao DQ, Zhang CY, Qui CP, Cai XZ, Ling CF, Song AH, Tang X, , 1995. Changes in the resistance of Plasmodium falciparum to chloroquine in Hainan, China. Bull World Health Organ 73: 483. [Google Scholar]
  26. Schwenke A, Brandts C, Philipps J, Winkler S, Wernsdorfer WH, Kremsner PG, , 2001. Declining chloroquine resistance of Plasmodium falciparum in Lambaréné, Gabon from 1992 to 1998. Wien Klin Wochenschr 113: 6364. [Google Scholar]
  27. Cortese JF, Caraballo A, Contreras CE, Plowe CV, , 2002. Origin and dissemination of Plasmodium falciparum drug resistance mutations in South America. J Infect Dis 186: 9991006.[Crossref] [Google Scholar]
  28. Pillai DR, Labbé AC, Vanisaveth V, Hongvangthong B, Pomphida S, Inkathone S, Zhong K, Kain KC, , 2001. Plasmodium falciparum malaria in Laos: chloroquine treatment outcome and predictive value of molecular markers. J Infect Dis 183: 789795.[Crossref] [Google Scholar]
  29. Huaman MC, Roncal N, Nakazawa S, Long TTA, Gerena L, Garcia C, Solari L, Magill AJ, Kanbara H, , 2004. Polymorphism of the Plasmodium falciparum multidrug resistance and chloroquine resistance transporter genes and in vitro susceptibility to aminoquinolines in isolates from the Peruvian amazon. Am J Trop Med Hyg 70: 461466. [Google Scholar]
  30. Jelinek T, Aida AO, Peyerl-Hoffmann G, Jordan S, Mayor A, Heuschkel C, el Valy AO, von Sonnenburg F, Christophel EM, , 2002. Diagnostic value of molecular markers in chloroquine-resistant falciparum malaria in Southern Mauritania. Am J Trop Med Hyg 67: 449453. [Google Scholar]
  31. Huaman MC, Yoshinaga K, Suryanatha A, Suarsana N, Kanbara H, , 2004. Short report: polymorphisms in the chloroquine resistance transporter gene in Plasmodium falciparum isolates from Lombok, Indonesia. Am J Trop Med Hyg 71: 4042. [Google Scholar]
  32. Ojurongbe O, Ogungbamigbe T, Fagbenro-Beyioku A, Fendel R, Kremsner P, Kun J, , 2007. Rapid detection of Pfcrt and Pfmdr1 mutations in Plasmodium falciparum isolates by FRET and in vivo response to chloroquine among children from Osogbo, Nigeria. Malar J 6: 41.[Crossref] [Google Scholar]
  33. Basco LK, Bras JL, Rhoades Z, Wilson CM, , 1995. Analysis of pfmdr1 and drug susceptibility in fresh isolates of Plasmodium falciparum from subsaharan Africa. Mol Biochem Parasitol 74: 157166.[Crossref] [Google Scholar]
  34. Fancony C, Gamboa D, Sebastiao Y, Hallett R, Sutherland C, Sousa-Figueiredo JC, Nery SV, , 2012. Various pfcrt and pfmdr1 Genotypes of Plasmodium falciparum Cocirculate with P. malariae, P. ovale spp., and P. vivax in northern Angola. Antimicrob Agents Chemother 56: 52715277.[Crossref] [Google Scholar]
  35. Mawili-Mboumba DP, Kun JFJ, Lell B, Kremsner PG, Ntoumi F, , 2002. Pfmdr1 alleles and response to ultralow-dose mefloquine treatment in Gabonese patients. Antimicrob Agents Chemother 46: 166170.[Crossref] [Google Scholar]
  36. Wootton JC, Feng X, Ferdig MT, Cooper RA, Mu J, Baruch DI, Magill AJ, Su X, , 2002. Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum . Nature 418: 320323.[Crossref] [Google Scholar]
  37. Roper C, Pearce R, Nair S, Sharp B, Nosten F, Anderson T, , 2004. Intercontinental spread of pyrimethamine-resistant malaria. Science 305: 1124.[Crossref] [Google Scholar]
  38. Adhin MR, Labadie-Bracho M, Vreden SG, , 2012. Status of potential PfATP6 molecular markers for artemisinin resistance in Suriname. Malar J 11: 322.[Crossref] [Google Scholar]
  39. Humphreys GS, Merinopoulos I, Ahmed J, Whitty CJM, Mutabingwa TK, Sutherland CJ, Hallett RL, , 2007. Amodiaquine and artemether-lumefantrine select distinct alleles of the Plasmodium falciparum mdr1 gene in Tanzanian children treated for uncomplicated malaria. Antimicrob Agents Chemother 51: 991997.[Crossref] [Google Scholar]
  40. Dokomajilar C, Nsobya SL, Greenhouse B, Rosenthal PJ, Dorsey G, , 2006. Selection of Plasmodium falciparum pfmdr1 alleles following therapy with artemether-lumefantrine in an area of Uganda where malaria is highly endemic. Antimicrob Agents Chemother 50: 18931895.[Crossref] [Google Scholar]
  41. Labadie-Bracho M, Adhin MR, , 2013. Increased pfmdr1 copy number in Plasmodium falciparum isolates from Suriname. Trop Med Int Health 18: 796799.[Crossref] [Google Scholar]
  42. Rogers WO, Sem R, Tero T, Chim P, Lim P, Muth S, Socheat D, Ariey F, Wongsrichanalai C, , 2009. Failure of artesunate-mefloquine combination therapy for uncomplicated Plasmodium falciparum malaria in southern Cambodia. Malar J 8: 10.[Crossref] [Google Scholar]
  43. Gadalla NB, Adam I, Elzaki S-E, Bashir S, Mukhtar I, Oguike M, Gadalla A, Mansour F, Warhurst D, El-Sayed BB, Sutherland CJ, , 2011. Increased pfmdr1 copy number and sequence polymorphisms in Plasmodium falciparum isolates from Sudanese malaria patients treated with artemether-lumefantrine. Antimicrob Agents Chemother 55: 54085411.[Crossref] [Google Scholar]
  44. Happi CT, Gbotosho GO, Folarin OA, Sowunmi A, Hudson T, O'Neil M, Milhous W, Wirth DF, Oduola AMJ, , 2009. Selection of Plasmodium falciparum multidrug resistance gene 1 alleles in asexual stages and gametocytes by artemether-lumefantrine in Nigerian children with uncomplicated falciparum malaria. Antimicrob Agents Chemother 53: 888895.[Crossref] [Google Scholar]
  45. Sisowath C, Strömberg J, Mårtensson A, Msellem M, Obondo C, Björkman A, Gil JP, , 2005. In vivo selection of Plasmodium falciparum pfmdr1 86N coding alleles by artemether-lumefantrine (Coartem). J Infect Dis 191: 10141017.[Crossref] [Google Scholar]
  46. Hastings IM, Ward SA, , 2005. Coartem (artemether-lumefantrine) in Africa: the beginning of the end? J Infect Dis 192: 13031304.[Crossref] [Google Scholar]
  47. Imwong M, Dondorp AM, Nosten F, Yi P, Mungthin M, Hanchana S, Das D, Phyo AP, Lwin KM, Pukrittayakamee S, Lee S, Saisung S, Koecharoen K, Nguon C, Day NP, Socheat D, White NJ, , 2010. Exploring the contribution of candidate genes to artemisinin resistance in Plasmodium falciparum . Antimicrob Agents Chemother 54: 28862892.[Crossref] [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.12-0695
Loading
/content/journals/10.4269/ajtmh.12-0695
Loading

Data & Media loading...

  • Received : 19 Nov 2012
  • Accepted : 22 May 2013
  • Published online : 07 Aug 2013

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error