1921
Volume 90, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

is an intracellular protozoan parasite found as sarcocysts within muscle fibers of intermediate hosts (monkey and baboon). The definitive host is suspected to be the snake. We report two cases from a larger cohort of 89 patients who had fever, headache, and generalized myalgia after a trip to Pangkor Island, Malaysia. Sarcocysts were detected in skeletal muscle biopsy specimens by light and electron microscopy from these two patients. DNA sequencing based on the 18S ribosomal DNA region identified the species as . We also identified sequences in the stools of a snake (). Phylogenetic analysis showed that these sequences form a cluster with most of the other known species for which the snake is a definitive host. We believe these two patients were likely to have symptomatic acute muscular sarcocystosis after infection that may have originated from snakes.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.12-0678
2014-02-05
2017-11-19
Loading full text...

Full text loading...

/deliver/fulltext/14761645/90/2/361.html?itemId=/content/journals/10.4269/ajtmh.12-0678&mimeType=html&fmt=ahah

References

  1. Fayer R, , 2004. Sarcocystis spp. in human infections. Clin Microbiol Rev 17: 894902.[Crossref]
  2. Kan SP, Prathap K, Dissanaike AS, , 1979. Light and electron microstructure of a Sarcocystis sp. from the Malaysian long-tailed monkey, Macaca fascicularis . Am J Trop Med Hyg 28: 634642.
  3. Wong K, Clarke G, Pathmanathan R, Hamilton P, , 1994. Light microscopic and three-dimensional morphology of the human muscular sarcocyst. Parasitol Res 80: 138140.[Crossref]
  4. Wong KT, Pathmanathan R, , 1994. Ultrastructure of the human skeletal muscle sarcocyst. J Parasitol 80: 327330.[Crossref]
  5. Fischer S, Odening K, , 1998. Characterization of bovine Sarcocystis species by analysis of their 18S ribosomal DNA sequences. J Parasitol 84: 5054.[Crossref]
  6. Yang ZQ, Zuo YX, Ding B, Chen XW, Luo J, Zhang YP, , 2001. Identification of Sarcocystis hominis-like (Protozoa: Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences. J Parasitol 87: 934937.[Crossref]
  7. Tian M, Chen Y, Wu L, Rosenthal BM, Liu X, He Y, Dunams DB, Cui L, Yang Z, , 2012. Phylogenetic analysis of Sarcocystis nesbitti (Coccidia: Sarcocystidae) suggests a snake as its probable definitive host. Vet Parasitol 183: 373376.[Crossref]
  8. Thomas V, Dissanaike AS, , 1978. Antibodies to Sarcocystis in Malaysians. Trans R Soc Trop Med Hyg 72: 303306.[Crossref]
  9. Wong KT, Pathmanathan R, , 1992. High prevalence of human skeletal muscle sarcocystosis in south-east Asia. Trans R Soc Trop Med Hyg 86: 631632.[Crossref]
  10. Arness MK, Brown JD, Dubey JP, Neafie RC, Granstrom DE, , 1999. An outbreak of acute eosinophilic myositis attributed to human Sarcocystis parasitism. Am J Trop Med Hyg 61: 548553.
  11. Husna Maizura AM, Khebir V, Chong CK, Azman Shah AM, Azri A, Lokman Hakim S, , 2012. Surveillance for sarcocystosis in Tioman Island, Malaysia. Malaysian J of Public Health Med 12: 3944.
  12. Centers for Disease Control and Prevention, 2012. Notes from the Field: Acute Muscular Sarcocystosis Among Returning Travelers—Tioman Island, Malaysia, 2011. Atlanta, GA: Centers for Disease Control and Prevention.
  13. Tamura K, Dudley J, Nei M, Kumar S, , 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 15961599.[Crossref]
  14. Pathmanathan R, Kan S, , 1981. Human Sarcocystis infection in Malaysia. Southeast Asian J Public Health Trop Med 12: 247250.
  15. Beaver PC, Gadgil K, Morera P, , 1979. Sarcocystis in man: a review and report of five cases. Am J Trop Med Hyg 28: 819844.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.12-0678
Loading
/content/journals/10.4269/ajtmh.12-0678
Loading

Data & Media loading...

  • Received : 05 Nov 2012
  • Accepted : 05 Aug 2013

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error