Volume 89, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Polymerase chain reaction was used to detect DNA from clinical samples collected from six leishmaniasis patients during 2011–2012. The samples used in this study came from bone marrow, blood, buffy coat, saliva, urine, and tissue biopsy specimens. Saliva was a good source for DNA by polymerase chain reaction. DNA was also found in saliva of an asymptomatic case-patient. Levels of DNA in saliva decreased until being undetectable after treatment. These levels could be used as a marker to evaluate efficacy of the treatment. A larger study is needed to evaluate this method as a screening and survey tool to study the silent background of infection among the at-risk population.


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  • Received : 02 Oct 2012
  • Accepted : 18 Aug 2013

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