1921
Volume 89, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The circulation of counterfeit or substandard artemisinins (ARTs) in malaria-endemic areas poses a serious threat to the long-term use of these drugs. Here, we validated an indirect competitive enzyme-linked immunosorbent assay (icELISA) for quantification of ARTs and found that 50% of inhibitory concentrations of dihydroartemisinin, artemether, and artesunate were 8.1, 207.0, and 4.7 ng/mL, respectively. We compared the icELISA with high-performance liquid chromatography (HPLC) for quantifying ART and its derivatives in 22 convenience samples of commercial antimalarial drugs. Paired tests showed a borderline significant difference between the two methods (mean = 0.03, 95% confidence interval [CI] 0.00–0.07, = 0.074) and the icELISA results were more variable than those of the HPLC analysis ( < 0.001), suggesting that further improvement is needed to enhance the performance of the icELISA. Our results showed that the icELISA has the potential to be improved for quality assurance of ARTs at the point of care in endemic settings.

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References

  1. Bates IF, Gruber J, Lalloo D, Medina Lara A, Squire SB, Theobald S, Thomson R, Tolhurst R, , 2004. Vulnerability to malaria, tuberculosis, and HIV/AIDS infection and disease. Part 1: determinants operating at individual and household level. Lancet Infect Dis 4: 267277.[Crossref] [Google Scholar]
  2. Vitoria M, Granich R, Gilks CF, Gunneberg C, Hosseini M, Were W, Raviglione M, De Cock KM, , 2009. The global fight against HIV/AIDS, tuberculosis, and malaria: current status and future perspectives. Am J Clin Pathol 131: 844848.[Crossref] [Google Scholar]
  3. WHO, 2010. World Malaria Report. Available at: http://www.who.int/malaria/world_malaria_report_2010/en/. Accessed September 25, 2013. [Google Scholar]
  4. Bassel GW, Holdsworth MJ, Provart NJ, , 2011. Seed bioinformatics. Methods Mol Biol 773: 403419.[Crossref] [Google Scholar]
  5. WHO, 2001. The Use of Antimalaria Drugs: Report of An Informal Consultation. (WHO/CDS/RBM/2001.33). Available at: http://rbm.who.int/cmc_upload/0/000/014/923/am_toc.htm/. Accessed September 25, 2013. [Google Scholar]
  6. Schlitzer M, , 2008. Antimalarial drugs—what is in use and what is in the pipeline. Arch Pharm Chem Life Sci 341: 149163.[Crossref] [Google Scholar]
  7. White NJ, , 2008. Qinghaosu (artemisinin): the price of success. Science 320: 330334.[Crossref] [Google Scholar]
  8. Wiesner JO, Jomaa H, Schlitzer M, , 2003. New antimalaria drugs. Angew Chem Int Ed Engl 42: 52745293.[Crossref] [Google Scholar]
  9. Newton PN, McGready R, Fernandez F, Green MD, Sunjio M, Bruneton C, Phanouvong S, Millet P, Whitty CJ, Talisuna AO, Proux S, Christophel EM, Malenga G, Singhasivanon P, Bojang K, Kaur H, Palmer K, Day NP, Greenwood BM, Nosten F, White NJ, , 2006. Manslaughter by fake artesunate in Asia–will Africa be next? PLoS Med 3: e197.[Crossref] [Google Scholar]
  10. Newton PN, McGready R, Fernandez F, Green MD, Sunjio M, Bruneton C, Phanouvong S, Millet P, Whitty CJ, Talisuna AO, Proux S, Christophel EM, Malenga G, Singhasivanon P, Bojang K, Kaur H, Palmer K, Day NP, Greenwood BM, Nosten F, White NJ, , 2006. Manslaughter by fake artesunate in Asia–will Africa be next? PLoS Med 3: 752755. [Google Scholar]
  11. WHO, 2006. Counterfeit Medicines. Fact Sheet No 275. Geneva: World Health Organization. [Google Scholar]
  12. Newton PN, Dondorp A, Green M, Mayxay M, White NJ, , 2003. Counterfeit artesunate antimalarials in Southeast Asia. Lancet 362: 169.[Crossref] [Google Scholar]
  13. Pea N, , 2001. Fake artesunate in Southeast Asia. Lancet 357: 19481950.[Crossref] [Google Scholar]
  14. Newton PN, Green MD, Fernandez F, , 2007. Counterfeit artemisinin derivatives and Africa: update from authors. PLoS Med 4: e139.[Crossref] [Google Scholar]
  15. Newton PN, Fernandez FM, Plancon A, Mildenhall DC, Green MD, Ziyong L, Christophel EM, Phanouvong S, Howells S, McIntosh E, Laurin P, Blum N, Hampton CY, Faure K, Nyadong L, Soong CW, Santoso B, Zhiguang W, Newton J, Palmer K, , 2008. A collaborative epidemiological investigation into the criminal fake artesunate trade in South East Asia. PLoS Med 5: e32.[Crossref] [Google Scholar]
  16. Keoluangkhot V, Green MD, Nyadong L, Fernandez FM, Mayxay M, Newton PN, , 2008. Impaired clinical response in a patient with uncomplicated falciparum malaria who received poor-quality and underdosed intramuscular artemether. Am J Trop Med Hyg 78: 552555. [Google Scholar]
  17. Bate R, Coticelli P, Tren R, Attaran A, , 2008. Antimalarial drug quality in the most severely malarious parts of Africa—a six country study. PLoS ONE 3: e2132.[Crossref] [Google Scholar]
  18. Woerdenbag HJ, Salomons MC, Visser JF, Hendriks H, Malingre TM, , 1991. Analysis of artemisinin and related sesquiterpenes from Artemisia annua L. by combined gas chromatography/mass spectrometry. Phytochem Anal 2: 215219.[Crossref] [Google Scholar]
  19. Acton NK, Rollman IJ, , 1985. Reductive electrochemical HPLC assay for artemisinin (Qinghaosu). Planta Med 51: 445446.[Crossref] [Google Scholar]
  20. Zhao S, Zeng M, , 1985. Studies on the analysis of qinghaosu by high-pressure liquid chromatograph and spectrometry (HPLC). Planta Med 51: 233237.[Crossref] [Google Scholar]
  21. Singh AV, Husain A, , 1988. Evaluation of Artemisia annua strains for higher artemisinin production. Planta Med 51: 233237. [Google Scholar]
  22. Peng CA, Wood AJ, , 2006. Direct analysis of artemisinin from Artemisia annua L. using high-performance liquid chromatography with evaporative light scattering detector, and gas chromatography with flame ionization detector. J Chromatogr A 1133: 254258.[Crossref] [Google Scholar]
  23. Banthorpe DV, , 1989. Two unexpected coumarin derivatives from tissue cultures of compositae species. Phytochemistry 28: 30033007.[Crossref] [Google Scholar]
  24. Woerdenbag HJ, Salomons MC, Hendriks H, Pras N, Malingre TM, , 1993. Volatile constituents of Artemisia annua L. (Asteraceae). Flav Frag 8: 131137.[Crossref] [Google Scholar]
  25. Wang MP, Wu Q, Simon JE, , 2005. Analysis of artemisinin in Artemisia annua L. by LC-MS with selected ion monitoring. J Agric Food Chem 53: 70107013.[Crossref] [Google Scholar]
  26. Song ZY, Liang XT, Liu CX, Yi MG, , 1985. Radio immunoassay of qinghaosu and artesunate. Acta Pharmacol Sin 20: 610614. [Google Scholar]
  27. Jaziri MD, Vanhaelen M, Homes J, Yoshimatsu K, Shimomura K, , 1993. Immunodetection of artemisinin in Artemisia annua cultivated in hydroponic conditions. Phytochemistry 33: 821826.[Crossref] [Google Scholar]
  28. Ferreira JF, , 1996. Immunoquantitative analysis of artemisinin from Artemisia annua using polyclonal antibodies. Phytochemistry 41: 97104.[Crossref] [Google Scholar]
  29. Tanaka HP, De-Eknamkul W, Matangkasombut O, Shoyama Y, , 2007. Preparation of a novel monoclonal antibody against the antimalaria drugs, artemisinin and artesunate. Planta Med 73: 11271132.[Crossref] [Google Scholar]
  30. Eggelte TA, van Agtmael M, Vuong TD, van Boxtel CJ, , 1999. The development of an immunoassay for the detection of artemisinin compounds in urine. Am J Trop Med Hyg 61: 449456. [Google Scholar]
  31. He SP, Li G, Tan WM, Nan TG, Wang BM, Li ZH, Li QX, , 2009. Development of a sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for the antimalaria active ingredient artemisinin in the Chinese herb Artemisinin annua L. Anal Bioanal Chem 393: 12971303.[Crossref] [Google Scholar]
  32. Wu JW, Shen WW, Zhang SQ, , 2009. HPLC with post-column derivatization for simultaneous determination of dihydroartemisinin and piperaquine. Chin Remedies Clinics 9: 178182. [Google Scholar]
  33. Cabri WD, Simone P, Di Iorio M, Di Mattia M, Gasparrini F, Giorgi F, Mazzanti A, Pierini M, Quaglia M, Villani C, , 2011. Stereolability of dihydroartemisinin, an antimalarial drug: a comprehensive kinetic investigation. Part 2. J Org Chem 76: 48314840.[Crossref] [Google Scholar]
  34. Newton P, Proux S, Green M, Smithuis F, Rozendaal J, Prakongpan S, Chotivanich K, Mayxay M, Looareesuwan S, Farrar J, Nosten F, White NJ, , 2001. Fake artesunate in Southeast Asia. Lancet 357: 19481950.[Crossref] [Google Scholar]
  35. Dondorp AM, Newton PN, Mayxay M, Van Damme W, Smithuis FM, Yeung S, Petit A, Lynam AJ, Johnson A, Hien TT, McGready R, Farrar JJ, Looareesuwan S, Day NP, Green MD, White NJ, , 2004. Fake antimalarials in Southeast Asia are a major impediment to malaria control: multinational cross-sectional survey on the prevalence of fake antimalarials. Trop Med Int Health 9: 12411246.[Crossref] [Google Scholar]
  36. Dorlo TP, Eggelte TA, Schoone GJ, de Vries PJ, Beijnen JH, , 2012. A poor-quality generic drug for the treatment of visceral leishmaniasis: a case report and appeal. PLoS Negl Trop Dis 6: e1544.[Crossref] [Google Scholar]
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  • Received : 20 Sep 2012
  • Accepted : 18 Jul 2013
  • Published online : 04 Dec 2013

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