Volume 88, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The analysis of promastigote excreted–secreted antigen (ESA) reactivity with 53 visceral leishmaniasis (VL) cases showed that each sample reacted regardless of the antigen or the species used in enzyme-linked immunosorbent assay (ELISA) displayed 100% positivity with the () ESA-blot recognizing bands of molecular weight ranging from 26.5 to 31.5 kDa. The analysis of 160 non-visceral cases showed that 5% of the samples cross-reacted with the () ESA-ELISA and 9.4% reacted with the ESA isolated from () and () , whereas a high cross-reaction ranging from 24.4% to 25% was observed with total crude promastigote antigens (PRO-ELISA). The ESA-blot of () tested with non-visceral sera samples showed a cross-reaction with 8.8% of cases; most of these cases represented tegumentary leishmaniasis and only one acute chagasic case. These data lead us to recommend the use of ESA as an alternative antigen in VL diagnosis.


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  • Received : 19 Sep 2012
  • Accepted : 09 Dec 2012
  • Published online : 06 Mar 2013

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