1921
Volume 88, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The analysis of promastigote excreted–secreted antigen (ESA) reactivity with 53 visceral leishmaniasis (VL) cases showed that each sample reacted regardless of the antigen or the species used in enzyme-linked immunosorbent assay (ELISA) displayed 100% positivity with the () ESA-blot recognizing bands of molecular weight ranging from 26.5 to 31.5 kDa. The analysis of 160 non-visceral cases showed that 5% of the samples cross-reacted with the () ESA-ELISA and 9.4% reacted with the ESA isolated from () and () , whereas a high cross-reaction ranging from 24.4% to 25% was observed with total crude promastigote antigens (PRO-ELISA). The ESA-blot of () tested with non-visceral sera samples showed a cross-reaction with 8.8% of cases; most of these cases represented tegumentary leishmaniasis and only one acute chagasic case. These data lead us to recommend the use of ESA as an alternative antigen in VL diagnosis.

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  • Received : 19 Sep 2012
  • Accepted : 09 Dec 2012
  • Published online : 06 Mar 2013

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