1921
Volume 89, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The Malaria Research and Reference Reagent Resource–recommended PLF/UNR/VIR polymerase chain reaction (PCR) was used to detect in spp. mosquitoes collected in South Korea. Samples that were amplified were sequenced and compared with known spp. by using the PlasmoDB.org Basic Local Alignment Search Tool/n and the National Center for Biotechnology Information Basic Local Alignment Search Tool/n tools. Results show that the primers PLF/UNR/VIR used in this PCR can produce uninterpretable results and non-specific sequences in field-collected mosquitoes. Three additional PCRs (PLU/VIV, specific for 18S small subunit ribosomal DNA; , specific for a nuclear repeat; and GDCW/PLAS, specific for the mitochondrial marker, ) were then used to find a more accurate and interpretable assay. Samples that were amplified were again sequenced. The PLU/VIV and assays showed cross-reactivity with non- spp. and an arthropod fungus (). The GDCW/PLAS assay amplified only spp. but also amplified the non-human specific parasite from an mosquito. Detection of in South Korea is a new finding.

[open-access] This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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2013-12-04
2017-09-23
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  • Received : 17 Sep 2012
  • Accepted : 03 Sep 2013

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