Volume 88, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



In endemic countries with soil-transmitted helminths mass drug administration with albendazole or mebendazole are being implemented as a control strategy. However, it is well known in veterinary helminths that the use of the same benzimidazole drugs can place selection on the β-tubulin gene, leading to resistance. Given the concern that resistance could arise in human soil-transmitted helminths, there is an urgent need to develop accurate diagnostic tools for monitoring resistance. In this study, we developed molecular assays to detect putative resistance genetic changes in , , and hookworms, and we optimized an egg hatch assay for the canine hookworm and applied it to . Both assays were tested on field samples. The molecular assays demonstrated their reproducibility and capacity to detect the presence of worms carrying putative resistance-associated genetic changes. However, further investigations are needed to validate our molecular and biological tests on additional field isolates.


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  • Received : 09 Aug 2012
  • Accepted : 01 Feb 2013
  • Published online : 05 Jun 2013

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