Volume 88, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



is the only species of known to cause cutaneous leishmanisis (CL) in Mali. We amplified DNA stored on archived Giemsa-stained dermal scraping slides obtained from self-referral patients with clinically suspected CL seen in the Center National d'Appui A La Lutte Contre La Maladie (CNAM) in Bamako, Mali, to determine if any other species were responsible for CL in Mali and evaluate its geographic distribution. Polymerase chain reaction (PCR) amplification was performed using a species-specific primer pair that can amplify DNA from , , , and parasites, possible causative agents of CL in Mali. was the only species detected in 41 microscopically confirmed cases of CL from five regions of Mali (Kayes, Koulikoro, Ségou, Mopti, and Tombouctou). These results implicate as the predominant, possibly exclusive species responsible for CL in Mali.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. Alvar J, Velez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M, the WHO Leishmaniasis Control Team; , 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS One 7: e35671.[Crossref] [Google Scholar]
  2. Imperato PJ, Diakite S, , 1969. Leishmaniasis in the Republic of Mali. Trans R Soc Trop Med Hyg 63: 236241.[Crossref] [Google Scholar]
  3. Imperato PJ, Coulibaly B, Togola T, , 1970. Leishmanin skin sensitivity in northwestern Mali. Acta Trop 27: 260265. [Google Scholar]
  4. Imperato PJ, Fofana B, Sow O, Diallo S, , 1974. Leishmanin skin sensitivity in the inland delta of the Niger. Trop Geogr Med 26: 303306. [Google Scholar]
  5. Keita S, Faye O, Ndiaye HT, Kore HD, , 2003. Epidemiology and clinical polymorphism of cutaneous leishmaniasis diagnosed at CNAM (Farmerly Marchoux Institute), Bamako (Mali). Mali Med 18: 2932. [Google Scholar]
  6. Oliveira F, Doubmia S, Anderson JM, Ousmane F, Souleymane SD, Troare P, Cisse M, Camara G, Tall K, Coulibaly CA, Samake S, Sissoko I, Traore B, Diallo D, Keita S, Fairhurst RM, Valenzuela JG, Kamhawi S, , 2009. Discrepant prevalence and incidence of Leishmania infection between two neighboring villages in central Mali based on leishmanin skin test surveys. PLoS Negl Trop Dis 3: e565.[Crossref] [Google Scholar]
  7. Garin JP, Peyramond D, Piens MA, Rioux JA, Godfrey DG, Lanotte G, Pratlong F, , 1985. Presence of Leishmania major Yakimoff and Schokhor, 1914 in Mali. Enzymatic identification of a strain of human origin. Ann Parasitol Hum Comp 60: 9394.[Crossref] [Google Scholar]
  8. Izri MA, Doumbo O, Belazzoug S, Pratlong F, , 1989. Presence of Leishmania major MON-26 in Mali. Ann Parasitol Hum Comp 64: 510511.[Crossref] [Google Scholar]
  9. Pratlong F, Dereure J, Ravel C, Lami P, Balard Y, Serres G, Lanotte G, Rioux JA, Dedet JP, , 2009. Geographical distribution and epidemiological features of Old World cutaneous leishmaniasis foci, based on the isoenzyme analysis of 1048 strains. Trop Med Int Health 14: 10711085.[Crossref] [Google Scholar]
  10. Motazedian H, Karamian M, Noyes HA, Ardehali S, , 2002. DNA extraction and amplification of Leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous leishmaniasis by PCR. Ann Trop Med Parasitol 96: 3134.[Crossref] [Google Scholar]
  11. Volpini AC, Marques MJ, Lopes dos Santos S, Machado-Coelho GL, Mayrink W, Romanha AJ, , 2006. Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years. Clin Microbiol Infect 12: 815818.[Crossref] [Google Scholar]
  12. Anders G, Eisenberger CL, Jonas F, Greenblatt CL, , 2002. Distinguishing Leishmania tropica and Leishmania major in the Middle East using the polymerase chain reaction with kinetoplast DNA-specific primers. Trans R Soc Trop Med Hyg 96 (Suppl 1): S87S92.[Crossref] [Google Scholar]
  13. Anderson JM, Samake S, Jaramillo-Gutierrez G, Sissoko I, Coulibaly CA, Traoré B, Soucko C, Guindo B, Diarra D, Fay MP, Lawyer PG, Doumbia S, Valenzuela JG, Kamhawi S, , 2011. Seasonality and prevalence of Leishmania major infection in Phlebotomus duboscqi Neveu-Lemaire from two neighboring villages in central Mali. PLoS Negl Trop Dis 10: e1139.[Crossref] [Google Scholar]
  14. Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL, , 2006. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol 44: 14351439.[Crossref] [Google Scholar]

Data & Media loading...

  • Received : 18 Jul 2012
  • Accepted : 11 Dec 2012
  • Published online : 06 Mar 2013

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error