Volume 88, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Japanese encephalitis virus (JEV) infection in mosquitoes was monitored in Vietnam from 2006 to 2008. A total of 15,225 mosquitoes, identified as 26 species in five genera were collected and 12,621 were grouped into 447 pools for examination of JEV infection by assays for cytopathic effects in C6/36 cells and by RT-PCR to detect flavivirus RNA. Three JEV strains were isolated from Giles collected in northern and southern Vietnam and two JEV strains were isolated from Theobald collected in the highlands of Vietnam. Genetic and phylogenetic analyses, based on complete E gene nucleotide sequences, revealed that the five JEV strains were classified into the genotype I group and six amino acid differences were found in these five strains. These results indicated that multiple JEV genotype I populations are circulating countrywide in Vietnam, transmitted by bites of their and .


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  • Received : 03 Jul 2012
  • Accepted : 20 Dec 2012
  • Published online : 03 Apr 2013

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