1921
Volume 88, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of , G1, and DNA derived from parasite tissue or canid fecal samples. Specificity using parasite tissue-derived DNA was found to be 100% except for primers that faintly detected DNA. Sensitivity of the three assays for DNA detection was between 2 and 10 pg. Ethanol precipitation of negative PCR fecal samples was used to eliminate false negatives and served to increase sensitivity as exemplified by an increase in detection from 0% to 89% of coproDNA using necropsy-positive fox samples.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.12-0331
2013-04-03
2020-08-14
Loading full text...

Full text loading...

/deliver/fulltext/14761645/88/4/795.html?itemId=/content/journals/10.4269/ajtmh.12-0331&mimeType=html&fmt=ahah

References

  1. McManus DP, Zhang W, Li J, Bartley PB, 2006. Echinococcosis. Lancet 362: 12951304.[Crossref]
    [Google Scholar]
  2. Li T, Ito A, Nakaya K, Qiu J, Nakao M, Zhen R, Xiao N, Chen X, Giraudoux P, Craig PS, 2008. Species identification of human echinococcosis using histopathology and genotyping in northwestern China. Trans R Soc Trop Med Hyg 102: 585590.[Crossref]
    [Google Scholar]
  3. Li T, Chen X, Zhen R, Qiu J, Qiu D, Xiao N, Ito A, Wang H, Giraudoux P, Sako Y, Nakao M, Craig PS, 2010. Widespread co-endemicity of human cystic and alveolar echinococcosis on the eastern Tibetan Plateau, northwest Sichuan/southeast Qinghai, China. Acta Trop 113: 248256.[Crossref]
    [Google Scholar]
  4. Xiao N, Qiu J, Nakao M, Li T, Yang W, Chen X, Schantz PM, Craig PS, Ito A, 2005. Echinococcus shiquicus n. sp., a taeniid cestode from Tibetan fox and plateau pika in China. Int J Parasitol 35: 693701.[Crossref]
    [Google Scholar]
  5. Bretagne S, Guillou JP, Morand M, Houin R, 1993. Detection of Echinococcus multilocularis DNA in fox feces using DNA amplification. Parasitology 106: 193199.[Crossref]
    [Google Scholar]
  6. Monnier P, Cliquet F, Aubert MF, Bretagne S, 1996. Improvement of a polymerase chain reaction assay for the detection of Echinococcus multilocularis DNA in fecal samples of foxes. Vet Parasitol 67: 185195.[Crossref]
    [Google Scholar]
  7. Cabrera M, Canova S, Rosenzvit M, Guarnera E, 2002. Identification of Echinococcus granulosus eggs. Diagn Microbiol Infect Dis 44: 2934.[Crossref]
    [Google Scholar]
  8. Dinkel A, Njoroge EM, Zimmermann A, Wälz M, Zeyhle E, Elmahdi IE, Mackenstedt U, Romig T, 2004. A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. Int J Parasitol 34: 645653.[Crossref]
    [Google Scholar]
  9. Trachsel D, Deplazes P, Mathis A, 2007. Identification of taeniid eggs in the feces from carnivores based on multiplex PCR using targets in mitochondrial DNA. Parasitology 134: 911920.[Crossref]
    [Google Scholar]
  10. Hüttner M, Siefert L, Mackenstedt U, Romig T, 2009. A survey of Echinococcus species in wild carnivores and livestock in East Africa. Int J Parasitol 39: 12691276.[Crossref]
    [Google Scholar]
  11. Abbasi I, Branzburg A, Campos-Ponce M, Abdel Hafez SK, Raoul F, Craig PS, Hamburger J, 2003. Copro-diagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence. Am J Trop Med Hyg 69: 324330.
    [Google Scholar]
  12. Štefanic' S, Shaikenov BS, Deplazes P, Dinkel A, Torgerson PR, Mathis A, 2004. Polymerase chain reaction for detection of patent infections of Echinococcus granulosus (“sheep strain”) in naturally infected dogs. Parasitol Res 92: 347351.[Crossref]
    [Google Scholar]
  13. van der Giessen JW, Rombout YB, Franchimont JH, Limper LP, Homan WL, 1999. Detection of Echinococcus multilocularis in foxes in The Netherlands. Vet Parasitol 82: 4957.[Crossref]
    [Google Scholar]
  14. Dinkel A, von Nickisch-Rosenegk M, Bilger B, Merli M, Lucius R, Romig T, 1998. Detection of Echinococcus multilocularis in the definitive host: coprodiagnosis by PCR as an alternative to necropsy. J Clin Microbiol 36: 18711876.
    [Google Scholar]
  15. Boufana BS, Campos-Ponce M, Naidich A, Buishi I, Lahmar S, Zeyhle E, Jenkins DJ, Combes B, Wen H, Xiao N, Nakao M, Ito A, Qiu J, Craig PS, 2008. Evaluation of three PCR assays for the identification of the sheep strain (Genotype 1) of Echinococcus granulosus in canid feces and parasite tissues. Am J Trop Med Hyg 78: 777783.
    [Google Scholar]
  16. Xiao N, Nakao M, Qiu J, Budke CM, Giraudoux P, Craig PS, Ito A, 2006. Short report: Dual infection of animal hosts with different Echinococcus species in the eastern Qinghai-Tibet plateau region of China. Am J Trop Med Hyg 75: 292294.
    [Google Scholar]
  17. von Nickisch-Rosenegk M, Silva-Gonzalez R, Lucius R, 1999. Modification of universal 12S rDNA primers for specific amplification of contaminated Taenia spp. (Cestoda) gDNA enabling phylogenetic studies. Parasitol Res 85: 819825.[Crossref]
    [Google Scholar]
  18. Craig PS, Rogan MT, Campos-Ponce M, 2003. Echinococcosis: disease, detection and transmission. Parasitology 127 (Suppl): S5S20.
    [Google Scholar]
  19. Budke CM, Campos-Ponce M, Wang Q, Torgerson PR, 2005. A canine purgation study and risk factor analysis for echinococcosis in a high endemic region of the Tibetan plateau. Vet Parasitol 127: 4955.[Crossref]
    [Google Scholar]
  20. McManus DP, 2002. The molecular epidemiology of Echinococcus granulosus and cystic hydatid disease. Trans R Soc Trop Med Hyg 96 (Suppl): S151S157.[Crossref]
    [Google Scholar]
  21. McManus DP, 2006. Molecular discrimination of taeniid cestodes. Parasitol Int 55 (Suppl): S31S37.[Crossref]
    [Google Scholar]
  22. Schneider R, Gollackner B, Edel B, Schmid K, Wrba F, Tucek G, Walochnik J, Auer H, 2008. Development of a new PCR protocol for the detection of species and genotypes (strains) of Echinococcus in formalin-fixed, paraffin-embedded tissues. Int J Parasitol 38: 10651071.[Crossref]
    [Google Scholar]
  23. Armua-Fernandez MT, Wonaka N, Sakurai T, Nakamura S, Gottstein B, Deplazes P, Phiri IGK, Katakura K, Oku Y, 2011. Development of PCR/dot blot assay for specific detection and differentiation of taeniid cestode eggs in canids. Parasitol Int 60: 8489.[Crossref]
    [Google Scholar]
  24. Yang YR, Rosenzvit MC, Zhang LH, Zhang JZ, McManus DP, 2005. Molecular study of Echinococcus in west-central China. Parasitology 131: 547555.[Crossref]
    [Google Scholar]
  25. Bart JM, Abdukader M, Zhang YL, Lin RY, Wang YH, Nakao M, Ito A, Craig PS, Piarroux R, Vuitton DA, Wen H, 2006. Genotyping of human cystic echinococcosis in Xinjiang, PR China. Parasitology 133: 571579.[Crossref]
    [Google Scholar]
  26. Rishi AK, McManus DP, 1987. Genomic cloning of human Echinococcus granulosus DNA: isolation of recombinant plasmids and their use as genetic markers in strain characterization. Parasitology 94: 369383.[Crossref]
    [Google Scholar]
  27. Mathis A, Deplazes P, Eckert J, 1996. An improved system for PCR-based specific detection of Echinococcus multilocularis eggs. J Helminthol 70: 219222.[Crossref]
    [Google Scholar]
  28. Mathis A, Deplazes P, 2006. Copro-DNA tests for diagnosis of animal taeniid cestodes. Parasitol Int 55 (Suppl): S87S90.[Crossref]
    [Google Scholar]
  29. Dyachenko V, Beck E, Pantchev N, Bauer C, 2008. Cost-effective method of DNA extraction from taeniid eggs. Parasitol Res 102: 811813.[Crossref]
    [Google Scholar]
  30. Boufana B, Qiu J, Chen X, Budke C, Campos-Ponce M, Craig PS, 2013. First report of Echinococcus shiquicus in dogs from Eastern Qinghai-Tibet plateau region, China. Acta Trop (In press).
    [Google Scholar]
  31. Yamasaki H, Allan JC, Sato MO, Nakao M, Sako Y, Nakaya K, Qiu D, Mamuti W, Craig PS, Ito A, 2004. DNA differential diagnosis of taeniasis and cysticercosis by multiplex PCR. J Clin Microbiol 42: 548553.[Crossref]
    [Google Scholar]
  32. Naidich A, McManus DP, Canova SG, Gutierrez AM, Zhang W, Guarnera EA, Rosenzvit MC, 2006. Patent and pre-patent detection of Echinococcus granulosus genotypes in the definitive host. Mol Cell Probes 20: 510.[Crossref]
    [Google Scholar]
  33. Al Sabi' MN, Kapel CM, Deplazes P, 2007. Comparative copro-diagnosis of Echinococcus multilocularis in experimentally infected foxes. Parasitol Res 101: 731736.[Crossref]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.12-0331
Loading
/content/journals/10.4269/ajtmh.12-0331
Loading

Data & Media loading...

  • Received : 21 May 2012
  • Accepted : 03 Dec 2012
  • Published online : 03 Apr 2013

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error