1921
Volume 88, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

is now known as the fifth species that can cause human malaria. The merozoite surface protein (MSP) has been reported to be potential target for vaccination and diagnosis of malaria. MSP-1 has been shown to be immunogenic and its T cell epitopes could mediate cellular immune protection. However, limited studies have focused on MSP-1. In this study, an approximately 28-kDa recombinant MSP-1 (pkMSP-1) was expressed by using an system. The purified pkMSP-1 reacted with serum samples of patients infected with (31 of 31, 100%) and non- malaria (27 of 28, 96.43%) by Western blotting. The pkMSP-1 also reacted with (25 of 31, 80.65%) and non- malaria sera (20 of 28, 71.43%) in an enzyme-linked immunosorbent assay (ELISA). Most of the non-malarial infection (49 of 52 in by Western blotting and 46 of 52 in the ELISA) and healthy donor serum samples (65 of 65 by Western blotting and ELISA) did not react with recombinant pkMSP-1.

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2013-05-01
2017-09-22
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  • Received : 20 Apr 2012
  • Accepted : 28 Jan 2013

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