Volume 88, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



This study deals with the underlying causes of failure of chloroquine in the treatment of infection in some malaria-endemic regions of India. Samples were collected from 141 patients in Purulia from March of 2007 to April of 2008. drug susceptibility tests, parasitic DNA isolation followed by polymerase chain reaction, and restriction fragment-length polymorphisms of different codons of the gene (76) and genes (86, 1042, and 1246) were assessed. The responses of 141 patients to chloroquine were determined. Prevalence of double (58.16%) mutation (86Y+1246Y) and some (14.89%) single mutations with triple mutation (76T+86Y+1042D+1246Y) were found. Interestingly, double mutation (86Y and 1246Y codons) was observed with the early treatment failure cases. These results show, for the first time in India that chloroquine resistance and chloroquine treatment failure were caused by double ( < 0.001) mutation.


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  • Received : 21 Dec 2011
  • Accepted : 11 Jun 2012
  • Published online : 01 May 2013

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