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The Technique and Results of a Complement Fixation Test for the Diagnosis of Infections with Endamoeba Histolytica

In previous communications (1, 2), the writer briefly described a complement fixation test which has been found useful in the diagnosis of infections with Endamoeba histolytica, the cause of amoebic dysentery, and has detailed the results obtained with this test. In this paper the technique of the test will be described, together with the results that have been obtained by its application in actual clinical practice, as well as experimentally, over a period of nearly two years, during which time many hundreds of individuals have been tested, the results being checked by microscopical and cultural examinations of the feces of the tested individuals, and by the clinical histories and symptoms of such individuals.

Technique of the Test. The technique of this complement fixation test is practically the same as that used as the standard method for performing the complement fixation test for syphilis in the United States Army laboratories, fully described in the writer's book, "The Wassermann Test" (3), with suitable modifications made necessary by the difference in the character of the antigen used in this test.