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Trichomonas in Tissue Cultures

1. Epithelium, fibroblasts, and sympathetic nerve fibers from the intestine and stomach of the embryonic chick were grown in Locke-Lewis.

2. Five different species of Trichomonas and one species of Pentatrichomonas were cultured and introduced into these tissue cultures by means of a Barber pipette.

3. The trichomonads usually remained in the lower part of the hanging drop, away from the tissue cells.

4. They frequently bombarded pieces of cells and masses of bacteria, pushing them about over the field.

5. Occasionally they attached themselves to living fibroblasts by means of their axostyles or crawled over them with their undulating membranes, without injuring the cells.

6. Trichomonads crawled along the edges of epithelial plates without causing them to contract.

7. Many trichomonads were watched as they crawled over plexuses of sympathetic nerves without injuring them.

From the above observations one seems justified in concluding that the various strains of Trichomonas studied do not mechanically injure the fibroblasts, epithelial cells, or sympathetic nerves when grown in tissue cultures.