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Survival of Leptospira Icteroides in Various Environments¹

A method of isolating and cultivating water leptospirae has been published recently by J. H. Bauer.² By filtering natural waters through Berkefeld cylinders V and N and incorporating the filtrate in a suitable culture medium, he was able to isolate a number of strains of leptospira.

By the same method, with minor modifications of the culture medium, we have been able to recover Leptospira icteroides from artificially inoculated stagnant water and human feces and also from mosquitoes which had fed on inoculated guinea pigs. Attempts to recover the organism from inoculated urine have so far been unsuccessful, but it was recovered from the unfiltered urine of an inoculated guinea pig. During this work, observations were made as to the length of time that Leptospira icteroides survived in the substances inoculated and in the mosquitoes. These studies were carried on in the laboratory of the West African Yellow Fever Commission of the Rockefeller Foundation in Lagos, Nigeria.

¹ The studies and observations on which this paper is based were conducted with the support and under the auspices of the International Health Division of the Rockefeller Foundation.

² Bauer, J. H.: A method for the isolation of leptospiras from water. Amer. Jour. Trop. Med., 1927, vii, 177.