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The Isolation and Cultivation of Herpetomonas Muscae-Domesticae

Previous attempts at cultivating many protozoa parasitic in the alimentary tract of insects have usually failed due to the inhibiting effect of the bacterial flora which is difficult to separate from the protozoa. A method is here described whereby a flagellate protozoan (Herpetomonas muscae-domesticae) parasitic in the alimentary tract of adult house flies can be freed from the intestinal flora and grown indefinitely in pure culture.

The best media and conditions for the cultivation of the parasite are described. A detailed study of the growth characters and of the living microörganism is presented, and by a study of fixed and stained preparations the morphology of the cultural forms is described and compared with the forms found in flies.

It is proven that the flagellate cultivated is actually Herpetomonas muscae-domesticae. Additional proof is furnished that the genus Herpetomonas is closely related to the genus Leishmania.

Received December 28, 1925.