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The Propagation of Japanese Encephalitis Virus in the Mosquito by Parenteral Introduction and Serial Passage¹

1. Propagation of the virus of Japanese encephalitis in Culex quinquefasciatus was demonstrated by parenteral injection and serial passage.

2. The emulsified whole bodies of the mosquitoes gave LD₅₀ titers of 6.5–8.4 when injected intracerebrally into mice.

3. Virus could be demonstrated in appreciable amount 24 hours after injection into the mosquito, and was present in relatively high concentration after 48 hours. Close to the maximum titer appeared to be reached on the third or fourth day and presumably there was no great change thereafter for as long as 17 days.

4. Mosquitoes which were injected parenterally with the virus transmitted it to suckling mice by bite after as little as 48 hours of incubation at 30°C.

¹ From the Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland. The opinions and assertions contained in this article are the private ones of the writer and are not to be construed as official or reflecting the views of the Navy Department.

² The writer wishes to acknowledge, with thanks, the technical assistance rendered by John I. Thomas, Chief Hospitalman, U. S. Navy.