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Observations on the Experimental Transmission of Japanese Encephalitis by Mosquitoes¹

1. The results demonstrate that mice infected by the bite of Culex quinquefasciatus develop a sufficient concentration of virus in the blood to produce a transmissible infection in mosquitoes which feed upon them.

2. Mice 3–5 days old when infected developed blood titers which ranged from 3.6 to 5.2 while those 7–12 days old had titers of 2.8–3.8. The mosquitoes transmitted the virus when infected by blood having titers of 2.8, 3.2, 4.5, and 5.2, respectively. The titer is expressed as the exponent of the reciprocal of the LD₅₀ dilution.

¹ From the Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland. The opinions and assertions contained in this article are the private ones of the writers and are not to be construed as official or reflecting the views of the Navy Department.

² Commander, MSC, USN.

³ Chief Hospitalman, USN.