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The Cultivation of Endamoeba Histolytica in a Defined Medium^1,2,

A defined medium has now been developed. A few of the conditions and constituents necessary to successful routine ameba culture in this medium have been studied. Many more considerations remain to be investigated, among them the important contribution of any type of accompanying flora. Final determination of the basic nutritional requirements of Endamoaeba histolytica, even in a defined medium, probably cannot be made until the mechanisms contributing to the growth of E. histolytica by other organisms have been elucidated.

¹ Supported in part by a grant from The Division of Research Grants and Fellowships of The United States Public Health Service. The use of certain facilities of The Allan Hancock Foundation of The University of Southern California is gratefully acknowledged.

² Presented before The American Society of Tropical Medicine, Memphis, Tennessee, November 7, 1949.

³ From The Department of Medical Microbiology and The Department of Medicine, University of Southern California School of Medicine, University Park, Los Angeles 7.