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A method for the isolation and identification of Shigella and Salmonella from fecal specimens has been described. For convenience in performance the procedure was set up under five main divisions; planting, screening, presumptive serology, biochemical confirmation and serological identification. The simple screening and presumptive serological tests adopted have been found to reduce to a minimum the number of organisms that require further biochemical and serological study for complete identification.
1 This paper is a modification of the lecture presented in the form of colored slides at the meeting of the Southern Branch of the American Public Health Association, April 14, 1948, New Orleans, Louisiana.
2 From the Bureau of Laboratories, Florida State Board of Health, Jacksonville, Florida.
3 The authors are grateful to Dr. P. R. Edwards for reviewing the manuscript and to Mr. Calvin Stucker for drawing Chart 1.
4 Bacteriologist, USPHS Communicable Disease Center, Veterinary Division, in charge Veterinary Public Health Division, Bureau of Laboratories, State Board of Health, Jacksonville, Florida.
5 Director of Laboratories, Florida State Board of Health.
6 Former Assistant Director of Laboratories, Florida State Board of Health. Present address: School of Aviation Medicine, Randolph Field, Texas.
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