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An In Vitro Method of Testing the Amebicidal Activity of a New Chemical Agent Using Balamuth Medium¹

1. An in vitro method of testing the amebicidal action of a chemical agent is described in detail. The results of this study show that flask cultures employing the Balamuth medium are very satisfactory for the uniform growth of trophozoites of E. histolytica in large numbers.

2. Comparative tests on four chemical agents were made. N-Methyl-Emetine-diiodomethylate, a new compound, possesses extremely low amebicidal action in vitro. These agents arranged in the order of their amebicidal action are emetine hydrochloride (1 part in 80,000), Iodochloroquine (1 part in 6,000), Chloroquine (1 part in 4,250) and N-Methyl-Emetine-diiodomethylate (1 part in 50).

3. Only an approximate estimation of the effectiveness of drugs as amebicides can be determined from in vitro studies.

4. N-Methyl-Emetine-diiodomethylate has very little amebicidal activity in vitro.

¹ From the Department of Medicine, University of Illinois.

² All experiments were performed by Basil Anastopoulos, M.D. in the laboratory of Carroll Birch, M.D.