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Purification of Hemoflagellate Cultures with Antibiotics¹

In 1944 Snow (1) treated unsuccessfully cutaneous leishmaniasis with penicillin. In 1945 Fulton (2) demonstrated that penicillin had no effect in vivo or in vitro on hemoflagellates. Horgan (3) in 1945 failed to obtain positive results in the treatment of two clinical cases of leishmaniasis. Neghme (4) in 1945 again failed to show any effect of penicillin on trypanosomes. We have not been able to find evidence in the literature that streptomycin was used in vivo or in vitro in hemoflagellate infections. Our object was to carry out further studies on the effect of penicillin, streptomycin and penicillin-streptomycin combinations.

Material and Methods. Leishmania donovani and Trypanosoma cruzi were cultured on Seneca medium (5). In the overlay of 5 cc. saline 5,000 and 1,000 units of penicillin or streptomycin, respectively, and 5,000 units each of penicillin and streptomycin together were dissolved. These culture tubes were inoculated from actively growing cultures of the hemoflagellates.

¹ Department of Urology, College of Physicians and Surgeons, Columbia University, New York City, and Schering Corporation, Bloomfield, New Jersey.