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Cultivation of Endamoeba Histolytica in Artificial Media from Cysts in Drinking Water Subjected to Chlorination^*

1. Experiments are reported on the cultivation of the cysts of Endamoeba histolytica following controlled exposure to varying strengths of residual chlorine in water.

2. In a number of instances the cysts remained viable after such treatment.

3. Chlorination of drinking water in practical amounts cannot be depended upon to destroy all of the cysts of E. histolytica.

^* This investigation was carried out at the Ninth Medical Service Detachment (Laboratory), near Chabua, Assam, whose commanding officer, Lt. Col. H. A. VanAuken, authorized the project.

¹ Major, Sn.C.

² T/4 Med. Dept.

³ T/4 Med. Dept.