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Although chiggers are severe pests in many regions of the world and are important vectors of disease in a large area of the Far East, satisfactory rearing methods for these mites have not been found. Rearing is peculiarly important for disease transmission studies with chiggers, since an individual mite feeds on a vertebrate host but once during its entire life and in but one stage, the larva. The disease organisms must, therefore, pass from the larva of one generation through all successive stages, including the egg, to the larva of the next generation. While there has not yet been achieved the goal of carrying through the cycle from larva to larva without serious mortality, the method described below may serve as a basis for the development of a satisfactory technique. In its present form, however, the method has been of great value in studying all stages of the life cycle and in obtaining the taxonomically important nymphs and adults from larval chiggers.
The author is much indebted to Dr. Herbert C. Clark, Director of the Gorgas Memorial Laboratory, for excellent facilities provided for this work, and to Major Marshall Hertig, Sn.C., and Captain G. B. Fairchild, Sn.C., for assistance and helpful suggestions.
1 The work described in this paper was done under a contract recommended by the Committee on Medical Research between the Office of Scientific Research and Development and the Gorgas Memorial Laboratory, Panama City, R de P.
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