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Survival Time of Trophozoites of Endamoeba Histolytica and Its Practical Significance in Diagnosis¹

1. Survival time of trophozoites of Endamoeba histolytica was determined upon exposure to varying environmental temperatures i.e., 5°C., 22°–25°C., and 37°C. and results reported.

2. On the basis of data obtained an attempt was made to reach a solution whereby we may be able to determine the duration of time and temperature at which a sample of stool can be left prior to examination without losing the characteristic morphology necessary for definite diagnosis.

3. Immediate examination of freshly passed stool is the prime requisite in the diagnosis of clinical amebiasis. However, the finding reported in this paper may serve as an aid in diagnosis in case there is a delay in examination or when an additional sample of stool may not be obtainable.

Received February 20, 1945.
¹ Read at the Fortieth Annual Meeting of the American Society of Tropical Medicine, at St. Louis, Missouri, November 14–16, 1944.