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The Insectary Rearing of Anopheles Quadrimaculatus¹

Since the report (1) of the establishment of a perpetuating colony of A. quadrimaculatus in an unheated outdoor insectary in Florida, we have given only a brief account (2) of our early insectary practices. Since then the Florida colony has been continuously maintained for nearly three years, while we have successfully adapted our methods to the highly artificial environment of an indoor installation in our laboratories at The Rockefeller Institute in New York. Such an insectary is an indispensable adjunct to a malaria therapy service, as a year round source of vigorous uninfected anopheline mosquitoes, while it also affords opportunities for research on entomological, parasitological, and epidemiological problems of malaria that have heretofore either been approached with difficulty or not at all.

Our practices were originally worked out with Anopheles quadrimaculatus, and they have been found equally applicable without modification to the successful maintenance of a colony of A. punctipennis.

¹ The studies and observations on which this paper is based were conducted with the support and under the auspices of the International Health Division of The Rockefeller Foundation.