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The Rôle of Solutes and Colloids in the Nutrition of Anopheline Larvae

Preliminary Report¹

1. Hexyl resorcinol, in 1:1000 dilution in 30 parts glycerin and 70 parts water, has proven a satisfactory disinfectant for Culicid eggs provided they are immersed in the liquid for a period of two minutes.

2. Anopheline larvae are able to utilize material in solution and colloids in suspension from normal pool water. This has been demonstrated by the use of Seitz-Werke filtered media and the dialysable and non-dialysable fractions of water from breeding habitats.

3. Larvae of Anopheles crucians and A. quadrimaculatus are able to live up to a period of eleven days and to make significant growth, under sterile conditions, in Seitz-Werke filtered water.

4. These larvae are unable, however, to develop in the dialysate of water from the same breeding pools.

5. In the case of the non-dialysable fraction of the same type of water it has been found that the larvae may live up to six days and make a slight significant growth, indicating that the normal water colloids may be of considerable importance in the nutrition of anopheline larvae.

¹ National Research Fellow in the Biological Sciences. From the Parasitology Laboratory, Department of Tropical Medicine, Tulane Medical School, New Orleans, La. Read at the twenty-seventh annual meeting of the American Society of Tropical Medicine, New Orleans, November 18–20, 1931.

The writer desires to express his appreciation of the kindly interest and assistance received from Dr. E. C. Faust and also the valuable advice from Dr. L. C. Scott in regard to the technique of dialysis.