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Technical Precautions Employed in Maintaining the Virus of Yellow Fever in Monkeys and Mosquitoes¹

The virulence of a strain of yellow fever has been maintained in monkeys and mosquitoes over a period of three and one-half years. The injection of infective blood regularly induces a fatal infection in monkeys. This degree of virulence greatly facilitates the interpretation of experimental observations.

Preservation of the virus by freezing permits considerable economy in time and in the use of nonkeys. For the study of some of the filterable viruses a freezing room is much more indispensable than an incubator. The drying of frozen virus is discouraged.

Infective mosquitoes can be handled in large numbers with a minimum of work and without the tedious necessity of recording the exact numbers of infected stock kept on hand. By the use of a special but simple type of mosquito trap, several mosquitoes may be removed at one time from a cage of infected ones without allowing an opportunity for any to escape.

In the course of three and one-half years, no laboratory infections have been acquired from either monkeys or mosquitoes. One member of the staff developed a mild atypical infection from the virus maintained in mice, at a time when he was not exposed in any way to the virulent strain maintained in mosquitoes and monkeys.

¹ This work was aided by generous grants from the DeLamar Mobile Research Fund.