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Field observations of anopheline biology, while capable of yielding very important information, are time consuming, have a limited scope, and only rarely offer opportunities for the employment of experimental methods. The development of a technique that will permit the successful cage rearing of anophelines for indefinite generations will be a decided advance.
Some degree of success has attended our efforts to rear generations of A. quadrimaculatus in a cage, and, it is with the hope of interesting others to apply these methods in the study of anophelines, that this account of our experience is prepared.
While this is a problem that has attracted our interest for many years, it was not until 1926 that any degree of success was secured. This warranted an expansion of efforts in the following season which we found created new difficulties. These required the whole of the year 1927 and part of 1928 season for their solution.
The studies and observations on which this paper is based were conducted with the support and under the auspices of the International Health Division of the Rockefeller Foundation.
1 From the International Health Division, Station for Field Studies in Malaria, Edenton, North Carolina.
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