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Am. J. Trop. Med. Hyg., 9(5), 1960, pp. 512-517
Copyright © 1960 by The American Society of Tropical Medicine and Hygiene

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Protein and Carbohydrate Complement Fixing Antigens of Trypanosoma Cruzi*

Earl H. Fife, Jr. AND John F. Kent{dagger}
Department of Serology, Division of Communicable Disease, Walter Reed Army Institute of Research, Washington, D. C.

In attempts to develop a specifically reactive complement fixing antigen, desiccated Trypanosoma cruzi rendered essentially lipid-free by extraction with anhydrous ether were extracted in buffered saline solution and the saline extract treated with chloroform and n-amyl alcohol under conditions considered to minimize deterioration. This yielded protein and carbohydrate antigens separable, respectively, in the gel and aqueous phase of extracts thus treated. Each showed a high degree of specific reactivity, as demonstrated in complement fixation tests of sera representing the homologous and several heterologous infections. The findings indicated that the protein fraction was the antigen of choice for diagnostic complement fixation tests.


* Presented in part before the American Society of Tropical Medicine and Hygiene, Philadelphia, Pa., Nov. 1, 1957.


{dagger} Present address: Division of Laboratories and Research, New York State Health Department, Albany 1.






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Copyright © 1960 by the American Society of Tropical Medicine and Hygiene.