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Isolation of Phlebotomus Fever Virus from Phlebotomus Papatasi^*

A total of 24,179 female Phlebotomus flies collected in suburban Cairo, Egypt, were examined for phlebotomus fever virus. Virus was recovered from 4 of 59 sandfly pools tested; one pool of Phlebotomus papatasi and 3 pools of unindentified Phlebotomus, presumably consisting solely of P. papatasi, yielded virus. All four isolates were identified by complement-fixation tests as the Sicilian type of virus. Wild-caught engorged P. papatasi flies were shown to contain substances which neutralize the Sicilian type of phlebotomus fever virus. Failure to demonstrate similar activity in laboratory-reared sandflies suggests that the active principle is antibody contained in ingested human blood.

^* The opinions and assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Navy Department or the naval services at large.