AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 82(1), 2010, pp. 35-39
doi:10.4269/ajtmh.2010.09-0284;
Copyright © 2010 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gómez-Couso, H.
Right arrow Articles by Ares-Mazás, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gómez-Couso, H.
Right arrow Articles by Ares-Mazás, E.
Related Collections
Right arrow Global Health
Right arrow Cryptosporidiosis

Thermal Contribution to the Inactivation of Cryptosporidium in Plastic Bottles during Solar Water Disinfection Procedures

Hipólito Gómez-Couso, María Fontán-Sainz, AND Elvira Ares-Mazás*
Laboratorio de Parasitología, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Santiago de Compostela, A Coruña, Spain

To determine the thermal contribution, independent of ultraviolet radiation, on the inactivation of Cryptosporidium parvum during solar water disinfection procedures (SODIS), oocysts were exposed for 4, 8, and 12 hours to temperatures recorded in polyethylene terephthalate bottles in previous SODIS studies carried out under field conditions. Inclusion/exclusion of the fluorogenic vital dye propidium iodide, spontaneous excystation, and infectivity studies were used to determine the inactivation of oocysts. There was a significant increase in the percentage of oocysts that took up propidium iodide and in the number of oocysts that excysted spontaneously. There was also a significant decrease in the intensity of infection elicited in suckling mice at the end of all exposure times. The results of the study demonstrate the importance of temperature in the inactivation of C. parvum oocysts during application of SODIS under natural conditions.

Received May 26, 2009. Accepted for publication July 14, 2009.

We thank Jordana María Fernández-Alonso for assistance with the study.

Financial support: This study was supported by the European Union (grant no. FP6-INCO-CT-2006-031650-SODISWATER).

Authors' address: Hipólito Gómez-Couso, María Fontán-Sainz, and Elvira Ares-Mazás, Laboratorio de Parasitología, Facultad de Farmacia, Campus Universitario Sur, 15782 Santiago de Compostela, A Coruña, Spain.

Reprint requests: Elvira Ares-Mazás, Laboratorio de Parasitología, Facultad de Farmacia, Campus Universitario Sur, 15782 Santiago de Compostela, A Coruña, Spain, E-mail: melvira.ares{at}usc.es.

*Address correspondence to Elvira Ares-Mazás, Laboratorio de Parasitología, Facultad de Farmacia, Campus Universitario Sur, 15782 Santiago de Compostela, A Coruña, Spain. E-mail: melvira.ares{at}usc.es






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2010 by the American Society of Tropical Medicine and Hygiene.