AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 81(5), 2009, pp. 817-824
doi:10.4269/ajtmh.2009.08-0595;
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Wei, Y.
Right arrow Articles by Qin, E.
PubMed
Right arrow Articles by Wei, Y.
Right arrow Articles by Qin, E.

Translational Regulation by the 3' Untranslated Region of the Dengue Type 2 Virus Genome

Yan Wei, Chengfeng Qin*, Tao Jiang, Xiaofeng Li, Hui Zhao, Zhongyu Liu, Yongqiang Deng, Ran Liu, Shuiping Chen, Man Yu, AND Ede Qin*
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of China

The role of the 3'untranslated region (UTR) of the dengue virus (DENV) genome during viral translation remains to be elucidated. We assessed the contribution of well-defined RNA elements in the 3'UTR of DENV-2 to viral translation using a virus-induced reporting gene system and deoxyribozymes (DRzs) targeting the 3'UTR of the DENV-2 genome. Results show that mRNAs carrying a deletion of repeated conserved sequence (RCS2)-CS2 are translated less efficiently than wild type mRNAs. However, mRNAs with a deletion of CS1-stem loop (SL) are translated more efficiently. Thus, CS1-SL and RCS2-CS2 may have different effects on translational regulation. Additionally, the translation-suppressing effect of CS1-SL or the SL element is further confirmed in DENV-2-infected cells using DRzs. Mutagenesis studies show that, rather than the secondary structure, nucleotides 10663–10677 and 10709–10723 are responsible for translational suppression of SL. Overall, our results demonstrate that sequences and elements within the DENV-2 3'UTR regulate viral translation.

Received November 12, 2008. Accepted for publication July 27, 2009.

Acknowledgments: We thank Professor Stuart G. Siddell (University of Bristol, Bristol, United Kingdom) for reviewing the manuscript and providing useful comments, and Dr. Chun’e Xu for technical assistance.

Financial support: This study was supported in part by grants from National Natural Science Foundation of China (no. 30600530 and no. 30770107).

* Address correspondence to Chengfeng Qin and Ede Qin, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No. 20 Dongda Street, Fengtai, Beijing 100071, People’s Republic of China. E-mail: cfqin{at}hotmail.com; qinede{at}sohu.com.

Authors’ address: Yan Wei, Chengfeng Qin, Tao Jiang, Xiaofeng Li, Hui Zhao, Zhongyu Liu, Yongqiang Deng, Ran Liu, Shuiping Chen, Man Yu, and Ede Qin, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No. 20 Dongda Street, Fengtai, Beijing 100071, People’s Republic of China.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2009 by the American Society of Tropical Medicine and Hygiene.