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Am. J. Trop. Med. Hyg., 81(3), 2009, pp. 538-539
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


Giardia intestinalis Assemblages A and B Infections in Nepal

Anjana Singh*, Lalitha Janaki, William A. Petri, Jr, AND Eric R. Houpt
Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia; Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal

 

ABSTRACT

Giardia intestinalis is comprised of two major genotypes, A and B, which may vary in their propensity to cause disease. We tested for the presence of these two genotypes in stool samples from patients with gastrointestinal symptoms in Nepal. A total of 1,096 clinical specimens were screened by microscopy, and 45 samples with G. intestinalis were identified. Giardia infection was confirmed in 35 of 45 samples by a Giardia specific real-time polymerase chain reaction (PCR) assay. Genotyping of the Giardia PCR product by restriction fragment length polymorphism indicated that 74% (26 of 35) were assemblage B, 20% (7 of 35) were assemblage A, and 6% (2 of 35) were mixed assemblages.

Received February 20, 2009. Accepted for publication June 2, 2009.

Acknowledgments: We thank Professor B. M. Pokharel (Microbiology Department, Tribhuvan University Teaching Hospital) and Dr. R. Pant (Department of Pathology, Chhauni Military Hospital) for providing stool specimens and Dr. Alenka H. Radojcic (Fulbright Visiting Professor to Nepal) for help in isolation of DNA.

Financial support: This work was supported by National Institutes of Health grants AI043596, AI075396, AI066973, and AI069598. Anjana Singh was supported by a Fulbright Scholar Program, Council for International Exchange of Scholars, postdoctoral fellowship at the University of Virginia and the Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.

* Address correspondence to Anjana Singh, Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal. E-mail: anjanas67{at}hotmail.com

Authors’ addresses: Anjana Singh, Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu, Nepal, E-mail: anjanas67{at}hotmail.com. Lalitha Janaki, William A. Petri Jr, and Eric R. Houpt, Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, VA 22908, E-mails: lj6t{at}virginia.edu, wap3g{at}virginia.edu, and erh6k{at}virginia.edu.






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