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Am. J. Trop. Med. Hyg., 81(3), 2009, pp. 516-518
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

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SHORT REPORT


Detection of Plasmodium knowlesi by Real-Time Polymerase Chain Reaction

N. Esther Babady, Lynne M. Sloan, Jon E. Rosenblatt, AND Bobbi S. Pritt*
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota

 

ABSTRACT

We previously developed a real-time polymerase chain reaction (PCR) assay for detection of the four Plasmodium species that infect humans. Recent studies have shown that natural transmission of the simian parasite Plasmodium knowlesi to humans occurs frequently in Southeast Asia. We have expanded our PCR assay to include detection of P. knowlesi.

Received January 15, 2009. Accepted for publication June 8, 2009.

Acknowledgments: This study was presented in part at the 57th Annual Meeting of the American Society for Tropical Medicine and Hygiene in New Orleans, LA, 2008 (Abstract 955).

* Address correspondence to Bobbi S. Pritt, Division of Clinical Microbiology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. E-mail: pritt.bobbi{at}mayo.edu

Authors’ address: N. Esther Babady, Lynne M. Sloan, Jon E. Rosenblatt, and Bobbi S. Pritt, Division of Clinical Microbiology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905.






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Copyright © 2009 by the American Society of Tropical Medicine and Hygiene.