AJTMH HINARI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am. J. Trop. Med. Hyg., 81(2), 2009, pp. 273-276
Copyright © 2009 by The American Society of Tropical Medicine and Hygiene

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Figueiredo, L. T. M.
Right arrow Articles by Padula, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Figueiredo, L. T. M.
Right arrow Articles by Padula, P.

Evaluation of an Enzyme-Linked Immunosorbent Assay Based on Araraquara Virus Recombinant Nucleocapsid Protein

Luiz Tadeu Moraes Figueiredo*, Marcos Lazaro Moreli, Alessandra Abel Borges, Glauciane Garcia de Figueiredo, Soraya Jabur Badra, Ivani Bisordi, Akemi Suzuki, Silvana Capria, AND Paula Padula
Virology Research Center, School of Medicine of the University of São Paulo in Ribeirão Preto, Ribeirão Preto, Brazil; Adolfo Lutz Institute, São Paulo, Brazil; National Institute for Infectious Diseases Dr. Carlos G. Malbrán, Buenos Aires, Argentina

Laboratory diagnosis of hantavirus cardiopulmonary syndrome (HCPS) in Brazil has been performed mostly by detection of IgM antibodies to recombinant antigen purified from Sin Nombre virus and Andes virus (ANDV). Recently, a recombinant nucleocapsid (rN) protein of Araraquara virus (ARAV), a Brazilian hantavirus, was obtained in Escherichia coli. To evaluate ARAV rN as antigen for antibody detection, serum samples from 30 patients from Argentina seropositive for hantavirus were tested. All samples were positive for IgG and IgM by enzyme-linked immunosorbent assay (ELISA) using either ARAV rN or ANDV rN antigens. In Brazil, six of 60 serum samples from patients with suspected HCPS (10%) were positive for IgM by ELISA using ARAV rN antigen and 7 were positive using ANDV rN antigen. For results obtained with 90 serum samples analyzed by IgM ELISA with ANDV rN antigen, the sensitivity of the IgM ELISA using ARAV rN antigen was 97.2%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 98.1%. The results show that ARAV rN is a suitable antigen for diagnosis of hantavirus infection in Brazil and Argentina.

Received November 3, 2008. Accepted for publication April 28, 2009.

Acknowledgment: We thank Dr. Eurico Arruda for reviewing the manuscript.

* Address correspondence to Luiz Tadeu Moraes Figueiredo, Virology Research Center, School of Medicine of the University of São Paulo in Ribeirão Preto, Av. Bandeirantes 3900, Ribeirão Preto, SP, 14049-900, Brazil. E-mail: ltmfigue{at}fmrp.usp.br

Authors’ addresses: Luiz Tadeu Moraes Figueiredo, Marcos Lazaro Moreli, Alessandra Abel Borges, Glauciane Garcia de Figueiredo, and Soraya Jabur Badra, Virology Research Center, School of Medicine of the University of São Paulo in Ribeirão Preto, Av. Bandeirantes 3900, Ribeirão Preto, SP, 14049-900, Brazil. Ivani Bisordi and Akemi Suzuki, Adolfo Lutz Institute, São Paulo, SP, Brazil. Silvana Capria and Paula Padula, National Institute for Infectious Diseases Dr. Carlos G. Malbrán, Buenos Aires, Argentina.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2009 by the American Society of Tropical Medicine and Hygiene.